Isolation and characterization of a lytic bacteriophage targeting the multidrug-resistant Staphylococcus aureus and its efficacy in a combination with thyme oil as biocontrol agent of S. aureus in chicken products

Document Type : Full paper (Original article)

Author

Department of Food control, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

10.22099/ijvr.2020.38083.5543

Abstract

Background: The emergence and spread of methicillin resistant Staphylococcus aureusin food industryhave led to using the alternative natural bioagents for controlling S. aureus in food. Aims: The current work aimed to isolate and characterize a lytic phage specific to S. aureus and evaluate its efficacy with thyme oil for controlling S. aureus growth in chicken fillets. Methods:Twenty S. aureus strains previously isolated from ready-to-eat chicken products were tested for antimicrobial susceptibility and used for phage isolation. Results: All S. aureus strains were multidrug-resistant (MDR). The isolatedphage (vB_SauM_CP9) belonged to the family Myoviridae and maintained its stability at pH (4-9) and temperature (30-70°C). The phage showed lytic activity on ten S. aureus strains and had a burst size (228 pfu/infected cell), latent period (45 min) and rise period (15 min). A combination of S. aureus phage MOI10+ thyme oil 1% caused a higher significant reduction (87.22%) to S. aureus in artificially inoculated chicken fillets than individual treatment with bacteriophage or thyme oil. Conclusion: To our knowledge, this is the first report to evaluate the efficacy of bacteriophage and thyme oil for controlling the growth of MDR S. aureus in the chicken products and recommending application of S. aureus phage and thyme oil combination in food industry to achieve food safety goals and consumer protection as well as mitigate the antimicrobial resistance crisis.

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