A primary occurrence of inclusion body hepatitis in absence of predisposing agents in commercial broilers in Iran: a case report

Document Type : Scientific report

Authors

1 Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

10.22099/ijvr.2020.36735.5360

Abstract

Background: Fowl adenoviruses (FAdVs) associated with certain clinical diseases including inclusion body hepatitis (IBH) have become of considerable importance in the poultry industry. Currently, an increasing number of IBH outbreaks in different parts of Iranian poultry industries is a growing concern. Infectious bursal disease virus (IBDV) or chicken infectious anemia virus (CIAV) have historically been incriminated as predisposing factors for FAdVs to cause IBH. Furthermore, some have speculated whether IBDV vaccine strains impact on IBH clinical manifestation. The present report assesses the potential predisposing role of IBDV, CIAV, and infectious bursal disease) IBD( vaccine strains for FAdVs in the course of an IBH occurrence in the field. Case description: 90000 day-old broiler chickens with the same parent source were housed, at 4 day-interval, in two commercial farms in Shiraz, Iran. Increased mortality with lesions of hepatitis, suggestive of IBH, started in the primitive farm right after blind prescription of IBD vaccine at the age of 12-days-old. Consequently, IBD vaccination was postponed for the apparently healthy chickens of the other farm in which chickens were monitored for the occurrence of IBH afterwards. Laboratory examination was followed by histopathology and polymerase chain reaction (PCR) on liver, cloacal bursa, and thymus samples to determine the involvement of FAdV, IBDV, and CIAV in the occurrence of the disease. Findings/treatment and outcome: No evidence was found to support the predisposing role of neither IBD vaccination nor IBDV/CIAV infection in this IBH occurence. The results also demonstrated a primary role of the FAdV-11 as a causal agent of the IBH occurrence. Conclusion: The findings suggest that certain FAdVs are pathogenic enough to primarily induce IBH in young broilers.

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Christensen, NH and Saifuddin, M (1989). A primary epidemic of inclusion body hepatitis in broilers. Avian Dis., 33: 622-630.
Fadly, AM; Winterfield, R and Olander, HJ (1976). Role of the bursa of Fabricius in the pathogenicity of inclusion body hepatitis and infectious bursal disease viruses. Avian Dis., 20: 467-477.
Gomis, S; Goodhope, R; Ojkic, D and Willson, P (2006). Inclusion body hepatitis as a primary disease in broilers in Saskatchewan, Canada. Avian Dis., 50: 550-555.
Helmboldt, CF and Frazier, MN (1963). Avian hepatic inclusion bodies of unknown significance. Avian Dis., 7: 446-450.
Hess, M (2013). Aviadenovirus infections. In: Swayne, DE; Glisson, JR; McDougald, LR; Nolan, LK; Suarez, DL and Nair, V (Eds.), Diseases of poultry. (13th Edn.), Ames, Wiley-Blackwell. PP: 290-300.
Hoerr, FJ (2010). Clinical aspects of immunosuppression in poultry. Avian Dis., 54: 2-15.
Hosseini, H and Morshed, R (2012). Molecular identification of fowl adenovirus associated with inclusion body hepatitis in Iran. Iran J. Virol., 6: 7-12.
Khodakaram-Tafti, A; Asasi, K and Namazi, F (2016). Clinicopathological characteristics of acute inclusion body hepatitis outbreak in broiler chickens in Iran. Bulg. J. Vet. Med., 19: 163-168.
Lim, TH; Lee, HJ; Lee, DH; Lee, YN; Park, JK; Youn, HN; Kim, MS; Youn, HS; Lee, JB; Park, SY; Choi, IS and Song, CS (2011). Identification and virulence characterization of fowl adenoviruses in Korea. Avian Dis.,
55: 554-560.
Lin, TL; Wu, CC; Rosenberger, JK and Saif, YM (1994). Rapid differentiation of infectious bursal disease virus serotypes by polymerase chain reaction. J. Vet. Diagn. Invest., 6: 100-102.
Meulemans, G; Boschmans, M; Van den Berg, TP and Decaesstecker, M (2001). Polymerase chain reaction combined with restriction enzyme analysis for detection and differentiation of fowl adenoviruses. Avian Pathol., 30: 655-660.
Morshed, R; Hosseini, H; Langeroudi, AG; Fard, MHB and Charkhkar, S (2017). Fowl adenoviruses D and E cause inclusion body hepatitis outbreaks in broiler and broiler breeder pullet flocks. Avian Dis., 61: 205-210.
Nakamura, K; Mase, M; Yamamoto, Y; Takizawa, K; Kabeya, M; Wakuda, T; Matsuda, M; Chikuba, T; Yamamoto, Y; Ohyama, T; Takahashi, K; Sato, N; Akiyama, N; Honma, H and Imai, K (2011). Inclusion body hepatitis caused by fowl adenovirus in broiler chickens in Japan, 2009-2010. Avian Dis., 55: 719-723.
Nateghi, E; Razmyar, J and Bassami, MR (2014). Molecular characterization of avian adenoviruses in Iranian broiler flocks. Iran. J. Vet. Res., 15: 164-167.
Reece, RL; Barr, DA; Grix, DC; Forsyth, WM; Condron, RJ and Hindmarsh, M (1986). Observations on naturally occurring inclusion body hepatitis in Victorian chickens. Aust. Vet. J., 63: 201-202.
Rosenberger, JK; Eckroade, RJ; Klopp, S and Krauss, WC (1974). Characterization of several viruses isolated from chickens with inclusion body hepatitis and aplastic anemia. Avian Dis., 18: 399-409.
Schachner, A; Matos, M; Grafl, B and Hess, M (2018). Fowl adenovirus-induced diseases and strategies for their control–a review on the current global situation. Avian Pathol., 47: 111-126.
Steer, PA; O’Rourke, D; Ghorashi, SA and Noormohammadi, AH (2011). Application of high-resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. Aust. Vet. J., 89: 184-192.
Steer, PA; Sandy, JR; O’Rourke, D; Scott, PC; Browning, GF; Noormohammadi, AH (2015). Chronological analysis of gross and histological lesions induced by field strains of fowl adenovirus serotypes 1, 8b and 11 in one-day-old chickens. Avian Pathol., 44: 106-113.
Todd, D; Mawhinney, KA and McNulty, MS (1992). Detection and differentiation of chicken anemia virus isolates by using the polymerase chain reaction. J. Clin. Microbiol., 30: 1661-1666.
Toro, H; Gonzalez, C; Cerda, L; Hess, M; Reyes, E and Geisse, C (2000). Chicken anemia virus and fowl adenoviruses: association to induce the inclusion body hepatitis/hydropericardium syndrome. Avian Dis., 44: 51-58.