Prevalence and antimicrobial resistance of Salmonella isolates from goose farms in Northeast China

Document Type : Full paper (Original article)

Authors

1 Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China

2 MSc Student in Basic Veterinary Medicine, Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China

10.22099/ijvr.2020.37804.5498

Abstract

Background: Salmonella is one of the most important enteric pathogenic bacteria that threatened poultry health. Aims: This study aimed to investigate the prevalence and antimicrobial resistance of Salmonella isolates in goose farms. Methods: A total of 244 cloacal swabs were collected from goose farms to detect Salmonella in Northeast China. Antimicrobial susceptibility, and resistance gene distribution of Salmonella isolates were investigated. Results: Twenty-one Salmonella isolates were identified. Overall prevalence of Salmonella in the present study was 8.6%. Among the Salmonella isolates, the highest resistance frequencies belonged to amoxicillin (AMX) (85.7%), tetracycline (TET) and trimethoprim/sulfamethoxazole (SXT) (81%), followed by chloramphenicol (CHL) (76.2%), florfenicol (FLO) (71.4%), kanamycin (KAN) (47.6%), and gentamycin (GEN) (38.1%). Meanwhile, only 4.8% of the isolates were resistant to ciprofloxacin (CIP) and cefotaxime (CTX). None of the isolates was resistant to cefoperazone (CFP) and colistin B (CLB). Twenty isolates (95%) were simultaneously resistant to at least two antimicrobials. Ten resistance genes were detected among which the blaTEM-1, cmlA, aac(6’)-Ib-cr, sul1, sul2, sul3, and mcr-1.1 were the most prevalent, and presented in all 21 isolates followed by tetB (20/21), qnrB (19/21), and floR (15/21). Conclusion: Results indicated that Salmonella isolates from goose farms in Northeast China exhibited multi-drug resistance (MDR), harboring multiple antimicrobial resistance genes. Our results will be useful to design prevention and therapeutic strategies against Salmonella infection in goose farms.

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Almeida, F; Seribelli, AA; Medeiros, MIC; Rodrigues, DDP; De Mellovarani, A; Luo, Y; Allard, MW and Falcao, JP (2018). Phylogenetic and antimicrobial resistance gene analysis of Salmonella Typhimurium strains isolated in Brazil by whole genome sequencing. PLoS One. 13: e0201882.
Amajoud, N; Bouchrif, B; El Maadoudi, M; Skalli Senhaji, N; Karraouan, B; El Harsal, A and El Abrini, J (2017). Prevalence, serotype distribution, and antimicrobial resistance of Salmonella isolated from food products in Morocco. J. Infect. Dev. Ctries., 11: 136-142.
Clinical and Laboratory Standards Institute (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. Document CLSI M7-A7, CLSI, Wayne.
Cui, M; Zhang, J; Gu, Z; Li, R; Chan, EW; Yan, M; Wu, C; Xu, X and Chen, S (2017). Prevalence and molecular characterization of mcr-1-positive Salmonella strains recovered from clinical specimens in China. Antimicrob. Agents. Chemother., 61: e02471-16.
Folster, JP; Campbell, D; Grass, J; Brown, AC; Bicknese, A; Tolar, B; Joseph, LA; Plumblee, JR; Walker, C; Fedorka-Cray, PJ and Whichard, JM (2015). Identification and characterization of multidrug-resistant Salmonella enterica serotype Albert isolates in the United States. Antimicrob. Agents. Chemother., 59: 2774-2779.
Gong, J; Zhang, J; Xu, M; Zhu, C; Yu, Y; Liu, X; Kelly, P; Xu, B and Wang, C (2014). Prevalence and fimbrial genotype distribution of poultry Salmonella isolates in China (2006 to 2012). Appl. Environ. Microbiol., 80: 687-693.
Gonzalez-Sanz, R; Herrera-Leon, S; De La Fuente, M; Arroyo, M and Echeita, MA (2009). Emergence of extended-spectrum beta-lactamases and ampc-type beta-lactamases in human Salmonella isolated in Spain from 2001 to 2005. J. Antimicrob Chemother. 64: 1181-1186.
Guard, J; Gast, RK and Guraya, R (2010). Colonization of avian reproductive-tract tissues by variant subpopulations of Salmonella Enteritidis. Avian Dis., 54: 857-861.
Igbinosa, IH (2015). Prevalence and detection of antibiotic-resistant determinant in Salmonella isolated from food-producing animals. Trop. Anim. Health Prod., 47: 37-43.
Lamas, A; Fernandez-No, IC; Miranda, JM; Vazquez, B; Cepeda, A and Franco, CM (2016). Prevalence, molecular characterization and antimicrobial resistance of Salmonella serovars isolated from Northwestern Spanish broiler flocks (2011-2015). Poult. Sci., 95: 2097-2105.
Liebana, E; Carattoli, A; Coque, TM; Hasman, H; Magiorakos, AP; Mevius, D; Peixe, L; Poirel, L; Schuepbach-Regula, G; Torneke, K; Torren-Edo, J; Torres, C and Threlfall, J (2013). Public health risks of enterobacterial isolates producing extended-spectrum beta-lactamases or ampc beta-lactamases in food and food-producing animals: An EU perspective of epidemiology, analytical methods, risk factors, and control options. Clin. Infect. Dis., 56: 1030-1037.
Liu, YY; Wang, Y; Walsh, TR; Yi, LX; Zhang, R; Spencer, J; Doi, Y; Tian, G; Dong, B; Huang, X; Yu, LF; Gu, D; Ren, H; Chen, X; Lv, L; He, D; Zhou, H; Liang, Z; Liu, JH and Shen, J (2016). Emergence of plasmid-mediated colistin resistance mechanism mcr-1 in animals and human beings in China: A microbiological and molecular biological study. Lancet Infect. Dis., 16: 161-168.
Livermore, DM (2003). Bacterial resistance: Origins, epidemiology, and impact. Clin. Infect. Dis., 36: S11-S23.
Lu, J; Quan, J; Zhao, D; Wang, Y; Yu, Y and Zhu, J (2019). Prevalence and molecular characteristics of mcr-1 gene in Salmonella Typhimurium in a tertiary hospital of Zhejiang province. Infect. Drug Resist., 12: 105-110.
Lunguya, O; Lejon, V; Phoba, MF; Bertrand, S; Vanhoof, R; Glupczynski, Y; Verhaegen, J; Muyembe-Tamfum, JJ and Jacobs, J (2013). Antimicrobial resistance in invasive non-typhoid Salmonella from the democratic republic of the congo: Emergence of decreased fluoroquinolone susceptibility and extended-spectrum beta lactamases. PLoS Negl. Trop. Dis., 7: e2103.
Magiorakos, AP; Srinivasan, A; Carey, RB; Carmeli, Y; Falagas, ME; Giske, CG; Harbarth, S; Hindler, JF; Kahlmeter, G; Olsson-Liljequist, B; Paterson, DL; Rice, LB; Stelling, J; Struelens, MJ; Vatopoulos, A; Weber, JT and Monnet, DL (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect., 18: 268-281.
Nang, SC; Li, J and Velkov, T (2019). The rise and spread of mcr plasmid-mediated polymyxin resistance. Crit. Rev. Microbiol., 45: 131-161.
Penha Filho, RAC; Ferreira, JC; Kanashiro, AMI; Berchieri Junior, A and Darini, A (2019). Emergent multidrug-resistant nontyphoidal Salmonella serovars isolated from poultry in Brazil coharboring blactx-m-2 and qnrb or blacmy-2 in large plasmids. Diagn. Microbiol. Infect. Dis., 95: 93-98.
Phongaran, D; Khang-Air, S and Angkititrakul, S (2019). Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand. Vet. World., 12: 1311-1318.
Quan, J; Li, X; Chen, Y; Jiang, Y; Zhou, Z; Zhang, H; Sun, L; Ruan, Z; Feng, Y; Akova, M and Yu, Y (2017). Prevalence of mcr-1 in Escherichia coli and Klebsiella pneumoniae recovered from bloodstream infections in China: A multicentre longitudinal study. Lancet Infect. Dis., 17: 400-410.
Rahn, K; De Grandis, SA; Clarke, RC; McEwen, SA; Galan, JE; Ginocchio, C; Curtiss , R and Gyles, CL (1992). Amplification of an inva gene sequence of Salmonella Typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol. Cell Probes. 6: 271-279.
Shah, DH; Paul, NC; Sischo, WC; Crespo, R and Guard, J (2017). Population dynamics and antimicrobial resistance of the most prevalent poultry-associated Salmonella serotypes. Poult. Sci., 96: 687-702.
Walsh, TR and Wu, Y (2016). China bans colistin as a feed additive for animals. Lancet Infect Dis., 16: 1102-1103.
Wang, J; Li, J; Liu, F; Cheng, Y and Su, J (2020). Characterization of Salmonella enterica isolates from diseased poultry in northern China between 2014 and 2018. Pathogens., 9: 95.
Yang, J; Gao, S; Chang, Y; Su, M; Xie, Y and Sun, S (2019). Occurrence and characterization of Salmonella isolated from large-scale breeder farms in Shandong province, China. Biomed. Res. Int., 2019: 8159567.
Yi, L; Wang, J; Gao, Y; Liu, Y; Doi, Y; Wu, R; Zeng, Z; Liang, Z and Liu, JH (2017). Mcr-1-harboring Salmonella enterica serovar Typhimurium sequence type 34 in pigs, China. Emerg. Infect. Dis., 23: 291-295.
Zhao, X; Yang, J; Zhang, B; Sun, S and Chang, W (2017). Characterization of integrons and resistance genes in Salmonella isolates from farm animals in Shandong province, China. Front Microbiol., 8: 1300.