1Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2MSc Student in Microbiology, Department of Medical Microbiology, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran
3Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Kruppel-like factors (Klfs) are a highly related zinc-finger family of transcription factors implicated in the regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. Using RT-PCR technique, a 456 bp cDNA fragment encoding N-terminus part of a Klf2b was isolated from the skin mucosa of common carp (Cyprinus carpio) using two degenerative oligonucleotide primers. Use of this fragment as a probe allowed the isolation of a larger cDNA clone through the searching of the GenBank expressed sequence tag database. The size of the amplified product is 1157 bp, which encodes a polypeptide of 274 amino acid residues with a predicted molecular mass of 30.359 kDa and theoretical pI of 4.88. The deduced amino acid sequence exhibited 79, 54, and 53% identity to the homologous Klf2b identified from zebrafish Danio rerio, Spotted Green Pufferfish Tetraodon nigroviridis and Atlantic salmon Salmo salar, respectively. The common carp protein is 50% similar to Klf2 orthologues in African clawed frog Xenopus laevis, 44% in chicken Gallus gallus and is 30% similar to the mammalian Klf in house mouse Mus musculus.