The emergence of Q1 genotype of Avian Infectious Bronchitis Virus in Iran, 2019: The first report

Document Type: Short paper

Author

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

10.22099/ijvr.2020.35858.5252

Abstract

Background: Avian Infectious Bronchitis (IB) is an infectious viral disease of chickens. The effective protection of chickens against many different IBV variants is not achieved unless identifying the circulating genotypes in the region and assessing the cross-protection of the potential of vaccines in use. Aims: In a monitoring program of IBVs, a new genotype was identified in the north of Iran, 2019.  This work was conducted to isolate and characterize this new IBV genotype. Methods: Tracheal tissues were collected from broilers showing signs of respiratory involvement. Specimens were homogenized and inoculated to the allantoic fluid of embryonated SPF eggs. IBV was detected using real-time PCR. The hypervariable region of the IBV S1 gene was amplified for sequencing. Results: Positive samples were phylogenetically analyzed, and both positive isolates were clustered with Q1 IBV strains. Conclusions: It is the first report of the Q1 outbreak in Iran. More investigations are needed to find the role of Q1 IVB in the respiratory disease complex of chickens.

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