1Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
3Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
In this study 130 bulk tank milk samples which were delivered to the Pegah Pasturisation Factory in Mashhad were collected randomly during the summer months. Samples were firstly enriched in modified trypticase soy broth containing novobiocin, followed by plating onto sorbitol MacConkey agar supplemented with cefixime and potassium tellurite for isolation of Escherichia coli O157:H7. Consequently the suspected non-sorbitol fermenting (NSF) colonies were confirmed by biochemical tests as Escherichia coli and then were used for multiplex-PCR assay, using primers specific for O157 and H7 antigens genes and then primers specific for stx1 and stx2 genes. NSF Escherichia coli colonies were recovered from 8 samples, and in multiplex-PCR assay one sample (0.77%) was confirmed as Escherichia coli O157:H7. The second multiplex PCR assay showed that the isolate was harboring the stx2 gene. The PCR assay used in this study may be a possible alternative to immunological assays which detect somatic and flagellar antigens. Besides, this procedure determines the potential of isolates for toxin production.