Molecular identification of aviadenoviruses in broiler chickens suspected to inclusion body hepatitis in Golestan province, Iran

Document Type: Short paper

Author

‍Ph.D. Student in Poultry Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

10.22099/ijvr.2020.34619.5113

Abstract

Background: Fowl adeno viruses( FADV) are distributed widely through out the word, and domestic avian species of all ages are susceptible.  Fowl aviadenoviruses (FAdV) can be separated in 5 different species (A–E) with various genotypes and 12 serotypes. Some geno‐ or serotypes induce hepatitis–hydropericardium syndrome (HHS), inclusion body hepatitis (IBH), and adenoviral gizzard erosion (AGE). Aims: Detect fowl adenoviruses serologically and molecularly and sequencing of  fowl adenoviruses in broiler flocks in Golestan province.  Methods: From December 2017 to June 2018 liver tissues and blood samples were collected from 31 broiler flocks suspected to IBH.  Polymerase chain reaction (PCR) was applied on liver samples and the positive samples were sequenced and antibody againstFADV was  measured by Elisa test . Results: Out of 31 flocks, the titers of 29 flocks (93.5%) were high in ELISA test for FAdV and 22 flocks (70.96%) were positive in PCR test. Sequence analysis indicated the isolates belonged to D and E genotype of Adenovirus. Conclusion: IBH-induced fowl adenoviruses are spreading increasingly in broiler flocks of Golestan province and needs more attention and surveillance programs of breeder and broiler farms to develop preventive measures and also vaccination of poultry farms in Iran should be considered by more complement studies.

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