Changes in Peripheral Blood Mononuclear Cells mRNA Expression of TLRs and CD14 during puerperal metritis in dairy cattle

Document Type: Full paper (Original article)

Author

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

10.22099/ijvr.2020.33748.5008

Abstract

Background: Peripheral blood mononuclear cells (PBMCs) are commonly referred to lymphocyte and monocyte, demonstrating the cells of adaptive and innate immune systems. Aims: Changes in PBMCs mRNA expression of pattern recognition receptors may be associated with puerperal metritis in Holstein cows. Methods: To test this hypothesis, PBMCs were collected from twenty cows with puerperal metritis and twenty cows without metritis at 10 days postpartum. Expression of Toll-like receptors (TLR4, TLR2), and cluster of differentiation 14 (CD14) genes were assessed in PBMCs using the quantitative Real Time-PCR technique. The data were normalized to the GAPDH as a reference gene, and 2-∆∆Ct methodology was used for relative quantification. Results: The results of our study demonstrated that the expression of TLR4 (P=0.04) and CD14 (P= 0.008) was significantly greater in cows with puerperal metritis compared to the control group. However, the expression of TLR2 (P=0.06) was not significantly different between cows with puerperal metritis and healthy cows. Conclusion: Our study suggests that puerperal metritis significantly increases the expression of TLR4 and CD14 genes in the PBMCs that contributes to proper stimulation of inflammation and consequently uterine clearance of bacteria soon after calving. 

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