Effect of prostaglandins E2 and F2α on granulosa cell apoptosis in goat ovarian follicles

Document Type: Full paper (Original article)

Authors

1 Ph.D. Student in Reproductive Physiology, Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra-136119, India

2 Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra University, Kurukshetra-136119, India

10.22099/ijvr.2020.35165.5175

Abstract

Background: The goat is a multi-purpose animal producing meat, milk, hide, fiber and manure, and hence plays a significant role in providing supplementary income and livelihood to the huge number of asset-poor ranchers and landless workers of rural India. Aims: The present study is aimed to investigate the in vitro effects of prostaglandins E2 (PGE2) and F (PGF) on ovarian granulosa cells of a goat. Methods: The healthy, slightly atretic and atretic antral follicles of goats with diameter ranging from 3-8 mm were cultured with PGE2 and PGF (1.0 μg/ml) along with control for 24 h. Histomorphological analysis was done in order to study the prostaglandins induced changes in granulosa cells of all the three categories of follicles. The acridine orange (AO) and methylene blue (MB) staining were used to study the apoptotic index in all the three categories of follicles and the collected data were analyzed by ANOVA with Duncan post Hoc test. Results: Prostaglandins E2 revealed positive while PGF showed a negative effect on the rescue of apoptosis in granulosa cells. The PGE2 treated follicles revealed a reduction in the attributes of apoptosis in granulosa cells while PGF showed an increased in the apoptotic characteristics. Conclusion: The present study depicted that granulosa cell viability in vitro is dependent on the continuous supply of survival and growth factors and prostaglandin of E series synthesis is a crucial step in the suppression of granulosa cell apoptosis while that of F series induces apoptosis.

Keywords


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