Effect of Prostaglandins E2 and F2α on Granulosa Cell Apoptosis in Goat Ovaries

Document Type: Full paper (Original article)


Reproductive Physiology Laboratory, Department of Zoology Kurukshetra University, Kurukshetra-136119, India



Background: The goat is a multi-purpose animal producing meat, milk, hide, fiber and manure, hence plays a significant role in providing supplementary income and livelihood to the huge number of asset poor ranchers and landless workers of rural India. Aims: The present study aimed to investigate the effect of prostaglandins E2 and F on cultured granulosa cells in the ovaries of a goat. Methods: The healthy, slightly atretic and atretic antral follicles of diameter ranging from 3-8 mm of goats were cultured with prostaglandin E2 and prostaglandin F2α (1.0 μg/ml) along with control for 24 hours. Histomorphological analysis was done in order to study the prostaglandins induced changes in granulosa cells of all the three categories of follicles. The acridine orange and methylene blue staining were used to study the apoptotic index in all the three categories of follicles and the data so obtained were analyzed by ANOVA with Duncan Post Hoc test. Results: PGE2 revealedpositive while PGF2α showed a negative effect on the rescue of apoptosis in granulosa cells. The PGE2 treated follicles revealed a reduction in the attributes of apoptosis in granulosa cells while PGF2α showed an increased in the apoptotic characteristics. Conclusion: The present study depicted that granulosa cell viability in vitro is dependent on the continuous supply of survival and growth factors and prostaglandin of E series synthesis is a crucial step in the suppression of granulosa cell apoptosis while of F series induces apoptosis.