Impact of omega-3 fatty acids preconception intake on some fertility parameters and foetuses quality of female rats

Document Type: Full paper (Original article)


Department of Zoology and Environmental Biology, University of Nigeria, Nsukka



Background / Aim: Researches have demonstrated that certain nutrients can reverse infertility hence the need for the present study “Impact of omega-3 fatty acids preconception intake on some fertility parameters of female rats and foetuses quality”. Methods: Thirty-four females and twelve male adult albino rats weighing 172-181 g were used. Firstly, twelve male rats were assigned into treatments 1, 2, 3 and 4 while twenty-four females were assigned into treatment 5, 6, 7 and 8. They were treated (administered orally): groups 1 and 5 (Controls) - 0.3 ml distilled water (placebo), groups 2 and 6 - 250 mg/kg O3FA, groups 3 and 7 - 25 mg/kg cyclophosphamide (CPP - negative control), while groups 4 and 8 received 25 mg/kg CPP + 250 mg/kg of O3FA for twenty-eight days. Thereafter, they were cohabitated (1:2 ratio male to female) into mating groups 1 to 7 until mating was confirmed. Ten pregnant rats (five of them were administered 500 mg/kg O3FA on days 15 and 16 of gestation) were used for abortifacient effect experiment. Results: Number of implantation sites, implantation index, percentage of pregnant females, life foetal number, foetal weight, foetal crown- rump length, corpora luteal number and fertility index of O3FA treated female rats and their foetuses significantly increased (P < 0.05) compared with negative control. No noticeable abortifacient effect occurred. Conclusion: Preconception intake of O3FA may have impacted female fertility positively.