Identification of Mycoplasma ovipneumoniae and Mycoplasma arginini in sheep with pneumonia in North East of Iran

Document Type: Full paper (Original article)

Authors

1 Ph.D. Student in Veterinary Pathology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 Division of Bacteriology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

Abstract

Background: Pneumonia due to Mycoplasma infections can cause serious health problems and economic losses in small ruminants industry. Aims: The aim of this study was isolation and identification of Mycoplasmas in sheep naturally infected with pneumonia in Northeastern Iran. Methods: This study used histopathology, culture and polymerase chain reaction (PCR) to examine samples from 50 lungs of sheep naturally infected with Mycoplasmas. Results: Grossly, irregular consolidation with lobular or lobar to diffuse pattern in the cranioventral to caudal lobes of affected lungs were observed. Histopathologically, bronchointerstitial pneumonia in 38 (76%), and purulent to fibrinopurulent bronchopneumonia in 12 (24%) affected sheep were diagnosed. DNA was extracted from lung tissue samples and replicated using genus and species specific primers for Mycoplasma. Mycoplasma growth was observed in 3 (6%) of a total of 50 lung samples. Genus-specific Mycoplasma DNA was identified by PCR in 12 (24%) of samples. Two (4%) and 7 (14%) samples of these 12 cases were positive for reaction with species-specific primers of Mycoplasma ovipneumoniae and Mycoplasma arginini, respectively. Conclusion: Our results showed that M. ovipneumoniae and M. arginini were the two agents that can be involved in inducing lung consolidation and pneumonia in sheep and PCR was more successful than the culture in detecting Mycoplasmas.
 

Keywords

Main Subjects


Abtin, AR; Pourbakhsh, SA; Ashtari, A; Bayatzadeh, MA; Barani, SM and Ahangaran, S (2013). Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran. Arch. Razi Inst., 68: 11-16.

Adehan, RK; Ajuwape, ATP; Adetosoye, AI and Alaka, OO (2006). Characterization of Mycoplasmas isolated from pneumonic lungs of sheep and goats. Small Rum. Res., 63: 44-49.

Amores, J; Corrales, JC; Martin, AG; Sanchez, A and Conteras, A (2010). Comparison of culture and PCR to detect Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in ear swabs taken from goats. Vet. Microbiol., 140: 105-108.

Ayling, RD; Bashiruddin, SE and Nicholas, RAJ (2004). Mycoplasma species and related organisms isolated from ruminants in Britain between 1999 and 2000. Vet. Rec., 155: 413-416.

Ayling, RD and Nicholas, RAJ (2007). Mycoplasma respiratory infections. In: Aitken, ID (Ed.), Diseases of sheep. (4th Edn.), Oxford, UK, Blackwell Publishing Inc., PP: 231-235.

Azizi, S; Tajbakhsh, E; Rezaii, SH; Nekouei, SH and Namjoo, AR (2011). The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in pneumonic lungs of slaughtered sheep. Rev. Med. Vet-Toulouse. 162: 310-315.

Carmichael, LE; St. George, TD; Sullivan, ND and Horsfall, N (1992). Isolation, propagation and characterization studies of an ovine Mycoplasma responsible for proliferative interstitial pneumonia. Cornell Vet., 62: 654-679.

Chazel, M; Tardy, F; Grand, DL; Calavas, D and Poumarat, F (2010). Mycoplasmoses of ruminants in France: recent data from the national surveillance network. BMC Vet. Res., 6: 32-39.

Cremonesi, P; Vimercati, C; Pisoni, G; Perez, G; Miranda Ribera, A; Castiglioni, B; Luzzana, M; Ruffo, G and Moroni, P (2007). Development of DNA extraction and PCR amplification protocols for detection of Mycoplasma bovis directly from milk samples. Vet. Res. Communi., (Suppl. 1), 31: 225-227.

Elfaki, MG; Abbas, B; Mahmoud, OM and Kleven, SH (2002). Isolation and characterization of Mycoplasma arginini from camels (Camelus dromedarius) with pneumonia. Comp. Immunol. Microbiol. Infect. Dis., 25: 49-57.

Ettorre, C; Sacchini, F; Scacchia, M and Salda, LD (2007). Pneumonia of lambs in the Abruzzo region of Italy: anatomopathological and histopathological studies and localization of Mycoplasma ovipneumoniae. Vet. Ital., 43: 149-155.

Fernandez, S; Galapero, J; Rey, J; Perez, CJ; Ramos, A; Rosales, R; Ayling, R; Alonso, JM and Gomes, L (2016) Investigations into the seasonal presence of Mycoplasma
species in fattening lambs. Vet. J., 212: 80-82.

Gagea, MI; Bateman, KG; Shanahan, RA; van Druemel, T; McEwen, BJ; Carman, S; Archambault, M and Caswell, JL (2006). Naturally occurring Mycoplasma bovis-associated pneumonia and polyarthritis in feedlot calves. J. Vet. Diagn. Invest., 18: 29-40.

Goncalves, R; Mariano, I; Núñez, A; Branco, S; Fairfoul, G and Nicholas, R (2010). Atypical non-progressive pneumonia in goats. Vet. J., 183: 219-221.

Gutierrez, C; Rodriguez, JL; Montoya, JA and Fernandez, A (1999). Clinicopathological and haematological findings in goat kids experimentally infected simultaneously with Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides (large colony-type). Small Rum. Res., 31: 187-192.

Haziroglu, R; Diker, KS; Gulbahar, MY; Akan, M and Guvenc, T (1994). Studies of the pathology and microbiology of pneumonic lungs of lambs. Dtsch. Tierarztl. Wochenschr., 101: 441-443.

Kheirkhah, B; Pourbakhsh, SA; Nadalian, MG; Banani, M and Ashtari, A (2011). Detection of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) methods from Iranian goats. Afr. J. Microbiol. Res., 5: 1668-1672.

Kılıc, A; Kalender, H; Eroksuz, H; Muz, A and Tasdemir, B (2013). Identification by culture, PCR, and immuno-histochemistry of Mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey. Trop. Anim. Health Prod., 45: 1525-1531. doi: 10.1007/s11250-013-0394-3.

Lopez, A and Martinson, SA (2017). Respiratory system, mediastinum and pleura. In: Zachery, JF (Ed.), Pathologic basis of veterinary disease. (6th Edn.), Vol. 1, Saint louis, Missouri, Elsevier Inc., PP: 538-539.

McAuliffe, L; Hatchell, FM; Ayling, RD; King, AI and Nicholas, RA (2003). Detection of Mycoplasma ovipneumoniae in Pasteurella-vaccinated sheep flocks with respiratory disease in England. Vet. Rec., 153: 687-688.

Mohan, K; Obowolo, MJ and Hill, PWG (1992). Mycoplasma ovipneumoniae infection in Zimbabwe goats and sheep. J. Comp. Pathol., 107: 73-79.

Niang, M; Rosenbusch, RF; Lopez-Virella, J and Kaeberle, ML (1999). Differential serologic response to Mycoplasma ovipneumoniae and Mycoplasma arginini in lambs affected with chronic respiratory disease. J. Vet. Diagn. Invest., 11: 34-40.

Pooladgar, AR; Looni, R; Ghaemmaghami, SH; Pourbakhsh, SA; Ashtari, A and Shirudi, A (2015). Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from affected sheep to contagious agalactia of Khuzestan province, Iran. Archives of Razi Institute. 70: 21-27.

Radostits, OM; Gay, CC; Hinchcliff, KW and Constable, PD (2007). Veterinary medicine. A textbook of the disease of cattle, horses, sheep, pigs and goats. 10th Edn., Baillier Tindall, London, Saunders Elsevier. PP: 950-995.

Smith, BP; Van Metre, DC and Pusterla, N (2019). Large animal internal medicine. 6th Edn., United States, Mosby Elsevier. PP: 645-698.

Tola, S; Angioi, A; Rocchigiani, G; Idini, D; Manunta, D; Galleri, G and Leori, G (1997). Detection of Mycoplasma agalactiae in sheep milk samples by polymerase chain reaction. Vet. Microbiol., 54: 17-22.