Molecular survey of avian circoviruses in some non-psittacine birds and detection of a novel canary circovirus in a pigeon

Document Type: Short paper

Authors

1 Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Avian Diseases Research Center, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

4 Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus, Ohio, America (current address)

Abstract

Background: Circoviruses are small, non-enveloped, single stranded DNA viruses. There is scarce information about these agents in non-psittacine birds. Aims: It is attempted to detect and characterize circoviruses in non-psittacine birds. Methods: Forty-five samples were collected from different non-psittacine species belonging to seven avian orders. A nested polymerase chain reaction (nested-PCR) for the detection of rep gene of circoviruses was applied. Results: Two different types of circoviruses were detected in two pigeon samples (2/11, 18.2%). One of the detected circoviruses was placed in clade A next to a polish strain based on phylogenetic analysis. Interestingly, the other detected circovirus was closely related to canary circoviruses (CaCVs). Conclusion: In addition to the molecular diagnosis of a pigeon circovirus (PiCV), this is the first report of the detection of CaCv in a pigeon. The possible hypotheses of such circumstance are discussed.

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