Molecular identification of reovirus in broiler type flocks in Golestan province, Iran

Document Type: Full paper (Original article)

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz; Ahvaz, Iran

2 Ph.D. Student in Poultry Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Departmet of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

Background: Avian reovirus (ARV) has a global distribution in nature and most clinical signs are found in broiler type chickens. Aims: This study was conducted to detect and identify reovirus infections from vaccinated breeder chickens and their progenies. Methods: A total of 20 tissue and blood samples were collected from vaccinated broiler breeders and their progenies with gastrointestinal or performance problems during peak production. Antibody titers were measured by indirect enzyme-linked immunosorbent assay (ELISA) tests. RNA extraction from tissue samples was performed and cDNA was prepared and directly used in the polymerase chain reaction (PCR). Nucleotide sequences were bilaterally determined using internal primers. The analysis of the nucleotide sequences and their related amino acids was performed by the specialized Molecular Evolutionary Genetics Analysis software (6th version). Results: The virus variant was detected in two vaccinated broiler breeders and five broiler flocks. The vaccine strains in breeder flocks included S1133, SS412, 1733, 2408 belonging to genotype 1 from the reovirus phylogenetic tree. Sequence 7 from the isolated reovirus based on the σC revealed that they were different from the reovirus vaccine, and that 6 isolates belonged to genotype 1 of the phylogenetic tree while 1 isolate belonged to branch 4 of the phylogenetic tree. Conclusion: The study showed that the new reovirus strain isolated from vaccinated birds differs from common strains used in the vaccines. It is therefore essential to prevent the effects of the field reovirus on the performance of industrial poultry, by updating and inventing new commercial vaccines, live and killed, against the reovirus.

Keywords


Bokaie, S; Shojadoost, B; Pourbaksh, SA; Pourseyyed, SM and Sharifi, L (2008). Seroprevalence survey on Reovirus infection of broiler chickens in Tehran province. Iran. J. Vet. Res., 9: 181-183.
Hedayati, M and Shojadoust, B (2012). Identification of tenosynovitis inducer reovirus in birds originating from Iranian breeder flocks by using RT-PCR & RFLP methods. Iranian J. Vet., 7: 135-142.
Hoseini, H; Bozorgmehrifard, M and Charkhkar, S (2015). Aredhal variants involves in reovirus-associated Arthritis and Tenosynovitis outbreaks in broiler flocks. 5th International Veterinary Poultry Congress. P: 58.
Huang, WR; Chiu, HC; Liao, TL; Chuang, KP; Shih, WL and Liu, HJ (2015). Avian reovirus protein P17. Functions as a nucleoporin Tpr suppressor leading to activation of p53, p21 and PTEN and inactivation of PI3K/AKT/mTOR and ERK signaling pathways. PLOS ONE. 10: e0138627.
Jones, RC (2009). Avian reovirus infection. OIE. 19: 614-625.
Jones, RC and Swane, DE (2013). Reovirus infection. In: Swane, DE; Glisson, JR; Mcdougald, LR; Nolan, LK; Suarez, DL and Nair, V (Eds.), Diseases of poultry. (13rd
Edn.), Ames, Iowa, Iowa State University Press. PP: 351-363.
Kant, A; Balk, F; Born, L; Van Roozelaar, D; Heijmans, J; Gielkens, A and Huurne,A (2003). Classification of Dutch and German avian reovirus by sequencing the Cσ protein. Vet. Res., 34: 203-212.
Kort, YH; Bourogâa, H; Gribaa, L; Hassen, J and Ghram, A (2013). Genotyping and classification of Tunisian strains of avian reovirus using RT-PCR and RFLP analysis. Avian Dis., 59: 14-19.
Lu, H (2015). Isolation and molecular characterization of newly emerging avian reovirus variants and novel strains in Pennsylvania, USA, 2011-2014. Sci. Rep., 5: Article No. 14727.
Mayahi, M; Khademian, S and Hoseini, H (2015). Report of reovirus infection in broiler farms from vaccinated breeders. 5th International Veterinary Poultry Congress. P: 159.
McNulty, MS; Jones, RC and Gough, RE (2008). Reoviridae. In: Pattison, M; McMulin, PF; Bradbury, JM and Alexander, DJ (Eds.), Poultry diseases. (6th Edn.), Canada, Toronto, Elsevier Publication Company. PP: 382-391.
Reck, C (2013). Rapid detection of Mycoplasma synovial and avian reovirus in clinical samples of poultry using multiplex PCR. Avian Dis., 57: 220-224.
Robertson, MD and Wilcox, GE (1989). Avian reovirus. Vet. Bull., 56: 155-174.
Rosenberger, K; Sterek, FF; Botts, K; Lee, KP and Margolin, A (1989). Invitro and Invivo characterization of avian reovirus. I. Pathogenicity and antigenic relatedness of several avian reovirus isolates. Avian Dis., 33: 535-544.
Senties-Cue, G; Shivaprasad, HL and Chin, RP (2005). Systemic Mycoplasma synovial infection in broiler chickens. Avian Pathol., 34: 137-142.
Spackman, E; Pantin-Jackwood, M; Day, JM and Sellers, H (2005). The pathogenesis of turkey origin reovirus in turkeys and chickens. Avian Pathol., 34: 291-296.
Tang, Y and Huaguang, L (2015). Genomic characterization of a broiler reovirus field strain detected in Pennsylvania. Infect. Genet. Evol., 31: 177-182.
Tang, Y; Lin, L; Knoll, EA; Dunn, PA; Wallner-Pendleton, EA and Lu, H (2015). The σC gene characterization of seven turkey arthritis reovirus field isolates in Pennsylvania during 2011-2014. J. Vet. Sci. Med., 3: 7.
Troxler, S (2013). Identification of a new reovirus causing substantial losses in broiler production in France, despite routine vaccination of breeders. Vet. Rec., 172: 556.