Application of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep

Document Type: Full paper (Original article)

Authors

1 Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Graduated from School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval
stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing,
serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for
 
epidemiologic studies. The present study was performed with a commercial human enzyme-linked
 
immunosorbent assay (ELISA) kit for the diagnosis of hydatidosis in sera collected from sheep with
 
hydatidosis. Sera were collected from 68 cases of hydatidosis proven by inspection of hydatid-infested livers
 
and lungs of the sheep slaughtered in Mashhad abattoir and also from 11 healthy cases. Sera samples were
 
examined by ELISA kit. The results showed that out of 68 cases of hydatidosis in sheep, 67 samples had
 
positive absorbance. Also from 11 healthy samples, 9 had negative absorbance value. The sensitivity and
 
specificity of the test were 99 and 82%, respectively. Therefore, it can be concluded that it is possible to use
 
human ELISA kit for the diagnosis of hydatidosis in sheep.
 
 

Keywords