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Alyethodi, R., Kumar, S., Deb, R., Alex, R., Singh, U., Sharma, S., Ashish, -., Choudhary, J., Sengar, G., Singh, R., Tyagi, S., Birham, P. (2018). Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India. Iranian Journal of Veterinary Research, 19(1), 44-47. doi: 10.22099/ijvr.2018.4767
R. R. Alyethodi; S. Kumar; R. Deb; R. Alex; U. Singh; S. Sharma; - Ashish; J. Choudhary; G. Sengar; R. Singh; S. Tyagi; P. Birham. "Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India". Iranian Journal of Veterinary Research, 19, 1, 2018, 44-47. doi: 10.22099/ijvr.2018.4767
Alyethodi, R., Kumar, S., Deb, R., Alex, R., Singh, U., Sharma, S., Ashish, -., Choudhary, J., Sengar, G., Singh, R., Tyagi, S., Birham, P. (2018). 'Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India', Iranian Journal of Veterinary Research, 19(1), pp. 44-47. doi: 10.22099/ijvr.2018.4767
Alyethodi, R., Kumar, S., Deb, R., Alex, R., Singh, U., Sharma, S., Ashish, -., Choudhary, J., Sengar, G., Singh, R., Tyagi, S., Birham, P. Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India. Iranian Journal of Veterinary Research, 2018; 19(1): 44-47. doi: 10.22099/ijvr.2018.4767

Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India

Article 9, Volume 19, Issue 1 - Serial Number 62, Winter 2018, Page 44-47  XML PDF (271 K)
Document Type: Short paper
DOI: 10.22099/ijvr.2018.4767
Authors
R. R. Alyethodi 1; S. Kumar1; R. Deb1; R. Alex1; U. Singh1; S. Sharma2; - Ashish2; J. Choudhary2; G. Sengar2; R. Singh1; S. Tyagi3; P. Birham1
1Animal Genetics and Breeding Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India
2MSc, Animal Genetics and Breeding Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India
3Animal Physiology Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India
Abstract
Complex vertebral malformation (CVM) has considerable economic impact on dairy cattle breeding due to extensive use of artificial insemination (AI). Identifying the carrier is an important factor to reduce the incidence of the genetic disorder. The study was conducted to identify the carriers of CVM in Frieswal cattle by polymerase chain reaction-primer-introduced restriction analysis (PCR-PIRA) method, which was further confirmed by sequencing. Carrier prevalence of 1% was observed in the Frieswal cattle. The results of the study clearly demonstrated the existence of carriers of CVM among Frieswal bull calves. Due to the widespread use of AI it is recommended to screen young bulls at early stages for this defective allele in order to avoid its rapid spread within the population.
Keywords
CVM; Frieswal; PCR-PIRA
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