Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India

Document Type: Short paper

Authors

1 Animal Genetics and Breeding Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India

2 MSc, Animal Genetics and Breeding Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India

3 Animal Physiology Section, ICAR-Central Institute for Research on Cattle, Meerut Cantt, Meerut, 250001, Uttar Pradesh, India

Abstract

Complex vertebral malformation (CVM) has considerable economic impact on dairy cattle breeding due to extensive use of artificial insemination (AI). Identifying the carrier is an important factor to reduce the incidence of the genetic disorder. The study was conducted to identify the carriers of CVM in Frieswal cattle by polymerase chain reaction-primer-introduced restriction analysis (PCR-PIRA) method, which was further confirmed by sequencing. Carrier prevalence of 1% was observed in the Frieswal cattle. The results of the study clearly demonstrated the existence of carriers of CVM among Frieswal bull calves. Due to the widespread use of AI it is recommended to screen young bulls at early stages for this defective allele in order to avoid its rapid spread within the population.

Keywords

References

Agerholm, JS (2007). Complex vertebral malformation syndrome in Holstein cattle: the story so far. Acta Vet. Scand., 49: S5.
Chu, Q; Sun, D; Yu, Y; Zhang, Y and Zhang, Y (2008). Identification of complex vertebral malformation carriers in Chinese Holstein. J. Vet. Diagn. Investig., 20: 228-230.
Chu, Q; Zhang, Y; Sun, DX; Yu, Y; Wang, YC and Zhang, Y (2010). Identification of the complex vertebral malformation gene in Chinese Holstein and its association with dairy performance traits. Yi Chuan (Hereditas). 32: 732-736.
Gabor, M; Miluchova, M; Trakovicka, A; Riecka, Z; Candrak, J and Vavrisinova, K (2012). Detection of complex vertebral malformation carriers in Slovak Holstein cattle by high resolution melting analysis. Acta Vet., 62: 239-248.
Kanae, Y; Daiji, E; Hajime, N and Masanobu, H (2005). A method for detecting complex vertebral malformation in Holstein’s calves using polymerase chain reaction–primer-introduced restriction analysis. J. Vet. Diagn. Invest., 17: 258-262.
Konersmann, Y; Wemheuer, W and Brenig, B (2003). Origin, distribution and relevance of the CVM defect within the Holstein-Friesian population. Zuchtungskunde. 75: 9-15.
Kotikalapudi, R; Patel, RK; Sunkara, PS and Roy, A (2013). Detection of silent homozygous polymorphism in exon 4 of SLC35A3 gene in a Holstein cattle carrier for complex vertebral malformation. Inter. J. Vet. Sci., 2: 61-64.
Mahdi, M; Ashwani, S; Dubey, PP; Kumar, V; Misra, B and Singh, A (2010). Identification of complex vertebral malformation using polymerase chain reaction–primer introduced restriction analysis in Karan Fries Bulls. Curr. Trends Biotechnol. Pharm., 4: 850-854.
Meydan, H; Yildiz, MA and Agerholm, JS (2010). Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinemia, and factor XI
deficiency in Holstein cows reared in Turkey. Acta Vet. Scand., 52: 1-8.
Nagahata, H; Hiromichi, O; Atsushi, N; Shin, O; Hidetoshi, H; Tetsuya, N; Takashi, K; Mitsuo, M and Hiroyuki, O (2002). Complex vertebral malformation in a stillborn Holstein calf in Japan. J. Vet. Med. Sci., 64: 1107-1112.
Nei, M (1987). Molecular evolutionary genetics. New York, ColumbiaUniversity Press. P: 512.
Rusc, A; Hering, D; Puckowska, P; Barcewicz, M and Kamiński, S (2013). Screening of Polish Holstein-Friesian bulls towards eradication of complex vertebral malformation (CVM) carriers. Pol. J. Vet. Sci., 16: 579-581.
Rusc, A and Kaminski, S (2007). Prevalence of complex vertebral malformation carriers among Polish Holstein Friesian bulls. J. Appl. Genet., 48: 247-252.
Sambrook, J; Fritschi, EF and Maniatis, T (1989). Molecular cloning: a laboratory manual. 2nd Edn., Cold Spring Harbor, NY: ColdSpringHarbor Laboratory. PP: 44-54.
Thomsen, B; Horn, P; Panitz, F; Bendixen, E; Petersen, AH; Holm, L; Nielsen, VH; Agerholm, JS; Arnbjerg, J and Bendixen, C (2006). A missense mutation in the bovine SLC35A3 gene, encoding a UDP-N-acetylglucosamine transporter, causes complex vertebral malformation. Genome Res., 16: 97-105.
Wang, C; Tong, Q; Hu, XZ; Yang, LG; Zhong, XQ; Yu, Y and Zhao, HQ (2011). Identification of complex vertebral malformation carriers in Holstein cattle in south China. Genet Mol. Res., 1: 2443-2448.
Zhang, Yi; Xuehua, F; Dongxiao, S; Yachun, W; Ying, Y; Yan, X; Shengli, Z and Yuan, Z (2012). A novel method for rapid and reliable detection of complex vertebral malformation and bovine leukocyte adhesion deficiency in Holstein cattle. J. Anim. Sci. Biotechnol., 3: 2-6.

Files

Share

How to cite

Statistics