LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples

Document Type: Short paper

Authors

1 Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut-250 001, Uttar Pradesh, India

2 MSc in Biotechnology, Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut-250 001, Uttar Pradesh, India

Abstract

Animal species detection is one of the crucial steps for consumer’s food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies.

Keywords


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