Co-relation of estrous cycle phases with uterine bacterial and fungal flora in non-pregnant female laboratory rabbits

Document Type: Short paper

Authors

1 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 Veterinary Medicine Private Practitioner, Shiraz, Iran

4 Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

This study was designed to investigate the relationship between the estrous cycle phases with uterine bacterial and fungal flora in non-pregnant female rabbits. Thirty laboratory mature multiparous rabbits were used for this purpose. Samples from uterine lavage for culture of bacteria and fungi were collected at different stages of estrous cycle (based on vaginal cytology), and histopathological observations were evaluated based on the scoring system used for defining the infection of the uterus. Various types of bacteria and fungi were isolated from rabbits at all stages of estrous cycle. The widest variety of bacteria and fungi was isolated at Di-estrous stage and the lowest variety was detected at estrous stage. Klebsiella oxytoca as well as yeast have been isolated at all stages of estrous cycle. This study showed that infection with K. oxytoca and yeast had no relationship with different stages of estrous cycle but other bacteria and fungus were associated with one or more stages of the estrous cycle in rabbits.

Keywords


Baba, E; Hata, H; Fukata, T and Arakawa, A (1983). Vaginal and uterine microflora of adult dogs. Am. J. Vet. Res., 44: 606-609.
Chengappa, MM; Maddux, RI and Grer, SC (1984). Isolation and identification of yeasts and yeastlike organisms from clinical veterinary sources. J. Clin. Microbiol., 19: 427-428.
Cleff, MB; Lima, AP; Faria, RO; Meinerz, ARM; Antunes, TA and Araujo, FB (2005). Isolation of Candida spp from vaginal microbiota of healthy canine females during estrous cycle. Br. J. Microbiol., 36: 201-204.
Devriese, LA (1984). A simplified system for biotyping Staphylococcus aureus strains isolated from different animal species. J. Appl. Bacteriol., 56: 215-220.
Garoussi, MT; Khosravi, AR and Havareshti, P (2007). Mycoflora of cervicovaginal fluids in dairy cows with or without reproductive disorders. Mycopathologia. 164: 97-100.
Jacques, M; Olson, ME; Crichlow, AM; Osborne, AD and Costerton, JW (1986). The normal microflora of the female rabbit’s genital tract. Can. J. Vet. Res., 50: 272-274.
Larsen, B; Markovetz, AJ and Galask, RP (1976). The bacterial flora of the female rat genital tract. Proc. Soc. Exp. Biol. Med., 151: 571-574.
Messier, S; Higgins, R; Couture, Y and Morin, M (1984). Comparison of swabbing and biopsy for studying the flora of the bovine uterus. Can. Vet. J., 25: 283-288.
Olson, PN and Mather, EC (1978). Canine vaginal and uterine bacterial flora. J. Am. Vet. Med. Assoc., 172: 708-711.
Oryan, A and Moshiri, A (2012). Recombinant fibroblast growth protein enhances healing ability of experimentally induced tendon injury in vivo. J. Tissue Eng. Regen. Med., 8: 421-423.
Oryan, A; Moshiri, A; Meimandi Parizi, AH and Raayat Jahromi, A (2012). Repeated administration of exogenous sodium-hyaluronate improved tendon healing in an in vivo transection model. J. Tissue Viability. 21: 88-102.
Schultheiss, PC; Jones, RL; Kesel, ML and Olson, PN (1999). Normal bacterial flora in canine and feline uteri. J. Vet. Diagn. Invest., 11: 560-562.
Scott, P; Daley, P; Baird, GG; Sturgess, S and Frost, AJ (1971). The aerobic bacterial flora of the reproductive tract of the mare. Vet. Rec., 88: 58-61.
Skangalis, M; Mahoney, CJ and O’leary, WM (1982). Microbial presence in the uterine cavity as affected by varieties of intrauterine contraceptive devices. Fertil. Steril., 37: 263-269.
Skangalis, M; Swenson, CE; Mahoney, CJ and O’Leary, WM (1979). The normal microbial flora of the baboon vagina. J. Med. Primatol., 8: 289-297.
Steel, C; Ratterree, M and Fidel, PL (1999). Differential susceptibility of two species of macaques to experimental vaginal candidiasis. J. Infec. Dis., 180: 802-810.
Tregier, A and Homburger, F (1961). Bacterial flora of the mouse uterus. Proc. Soc. Exp. Biol. Med., 108: 152-154.
Viana, D; Selva, L; Callanan, JJ; Guerrero, I; Ferrian, S and Corpa, JM (2011). Strains of Staphylococcus aureus and pathology associated with chronic suppurative mastitis in rabbits. Vet. J., 190: 403-407.
Watts, JR; Wright, PJ and Whithear, KC (1996). Uterine, cervical and vaginal microflora of the normal bitch throughout the reproductive cycle. J. Small Anim. Pract.,
37: 54-60.
Westwood, FR (2008). The female rat reproductive cycle: a practical histological guide to staging. Toxic Pathol., 36: 375-384.
Ypsilantis, P; Tsiligianni, Th and Karagiannidis, A (1996). The use of cytological examination of vaginal smears for the determination of domestic rabbit’s estrous cycle. Bull Hellenic Vet. Med. Soc., 47: 186-190.