VacA and cagA genotypes status and antimicrobial resistance properties of Helicobacter pylori strains isolated from meat products in Isfahan province, Iran

Document Type: Full paper (Original article)

Authors

1 Ph.D. Student, Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Food Hygiene, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Abstract

Although Helicobacter pylori has a significant impact on the occurrence of severe clinical syndromes, its exact ways of transmission and origin have not been identified. According to the results of some previously published articles, foods with animal origins play a substantial role in the transmission of H. pylori to humans. The present investigation was carried out to study the vacuolating cytotoxin A (vacA) and cytotoxin associated gene A (cagA) genotypes status and antibiotic resistance properties of H. pylori strains recovered from minced-meat and hamburger samples. A total of 150 meat product samples were collected from supermarkets. All samples were cultured and the susceptive colonies were then subjected to nested-PCR, PCR-based genotyping and disk diffusion methods. 11 out of 150 samples (7.33%) were positive for H. pylori. All the isolates were further identified using the nested-PCR assay. Prevalence of H. pylori in hamburger and minced-meat samples was 1.42% and 12.5%, respectively. S1a, m1a and cagA were the most commonly detected genotypes. The most commonly detected combined genotypes in the H. pylori strains of minced-meat were s1am1a (10%), s1am1b (10%) and s2m1a (10%). Helicobacter pylori strains of meat products harbored the highest levels of resistance against ampicillin (90.90%), erythromycin (72.72%), amoxicillin (72.72%), trimethoprim (63.63%), tetracycline (63.63%), and clarithromycin (63.63%). Hamburger and minced-meat samples may be the sources of virulent and resistant strains of H. pylori. Meat products are possible sources of resistant and virulent strains of H. pylori similar to those vacA and cagA genotypes. Using healthy raw materials and observation of personal hygiene can reduce the risk of H. pylori in meat products.

Keywords


Alikhani, MY; Arebestani, MR; Khorasani, MS; Majlesi, A and Jaefari, M (2014). Evaluation of Helicobacter pylori vacA and cagA genotypes and correlation with clinical outcome in patients with dyspepsia in Hamadan province, Iran. Iran. Red. Crescent. Med. J., 16: e19173.

Angelidis, AS; Tirodimos, I; Bobos, M; Kalamaki, MS; Papageorgiou, DK and Arvanitidou, M (2011). Detection of Helicobacter pylori in raw bovine milk by fluorescence in situ hybridization (FISH). Int. J. Food. Microbiol., 151: 252-256.

Atapoor, S; Dehkordi, FS and Rahimi, E (2014). Detection of Helicobacter pylori in various types of vegetables and salads. Jundishapur. J. Microbiol., 7: e10013.

Brown, LM (2000). Helicobacter pylori: epidemiology and routes of transmission. Epidemiol. Rev., 22: 283-297.

Chomvarin, C; Namwat, W; Chaicumpar, K; Mairiang, P; Sangchan, A; Sripa, B; Tor-Udom, S and Vilaichone, RK (2008). Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int. J. Infect. Dis., 12: 30-36.

De Francesco, V; Giorgio, F; Hassan, C; Manes, G; Vannella, L; Panella, C; Ierardi, E and Zullo, A (2010). Worldwide H. pylori antibiotic resistance: a systematic. J. Gastrointestin. Liver Dis., 19: 409-414.

Dore, MP; Sepulveda, AR; El-Zimaity, H; Yamaoka, Y; Osato, MS; Mototsugu, K; Nieddu, AM; Realdi, G and Graham, DY (2001). Isolation of Helicobacter pylori from sheep—implications for transmission to humans. Am. J. Gastroenterol., 96: 1396-1401.

Dunn, BE; Cohen, H and Blaser, MJ (1997). Helicobacter pylori. Clin. Microbiol. Rev., 10: 720-741.

Ghorbani, F; Gheisari, E and Safarpoor Dehkordi, F (2016). Genotyping of vacA alleles of Helicobacter pylori strains recovered from some Iranian food items. Trop. J. Pharm. Res., 15: 1631-1636.

Havaei, SA; Mohajeri, P; Khashei, R; Salehi, R and Tavakoli, H (2014). Prevalence of Helicobacter pylori vacA different genotypes in Isfahan, Iran. Adv. Biomed. Res., 3: 48.

Hemmatinezhad, B; Momtaz, H and Rahimi, E (2016). VacA, cagA, iceA and oipA genotypes status and anti-microbial resistance properties of Helicobacter pylori isolated from various types of ready to eat foods. Ann. Clin. Microbiol. Antimicrob., 15: 2.

Herrera, AG (2004). Helicobacter pylori and food products: a public health problem. Methods Mol. Biol., 268: 297-301.

Kusters, JG; van Vliet, AH and Kuipers, EJ (2006). Pathogenesis of Helicobacter pylori infection. Clin. Microbiol. Rev., 19: 449-490.

Mansour, KB; Fendri, C; Zribi, M; Masmoudi, A; Labbene, M; Fillali, A; Mami, NB; Najjar, T; Meherzi, A and Sfar, T (2010). Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients. Ann. Clin. Microbiol. Antimicrob., 9: 10.

Mhaskar, RS; Ricardo, I; Azliyati, A; Laxminarayan, R; Amol, B; Santosh, W and Boo, K (2013). Assessment of risk factors of Helicobacter pylori infection and peptic ulcer disease. J. Glob. Infect. Dis., 5: 60-67.

Miftahussurur, M; Sharma, RP; Shrestha, PK; Suzuki, R; Uchida, T and Yamaoka, Y (2015). Molecular epidemio-logy of Helicobacter pylori infection in Nepal: specific ancestor root. Plos. One. 10: e0134216.

Momtaz, H; Dabiri, H; Souod, N and Gholami, M (2014). Study of Helicobacter pylori genotype status in cows, sheep, goats and human beings. BMC. Gastroenterol., 14: 61.

Momtaz, H; Souod, N; Dabiri, H and Sarshar, M (2012). Study of Helicobacter pylori genotype status in saliva, dental plaques, stool and gastric biopsy samples. World J. Gastroenterol., 18: 2105-2111.

Mousavi, S; Dehkordi, FS and Rahimi, E (2014). Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran. J. Venom. Anim. Toxins. Incl. Trop. Dis., 20: 51.

Nagiyev, T; Yula, E; Abayli, B and Koksal, F (2009). Prevalence and genotypes of Helicobacter pylori in gastric biopsy specimens from patients with gastroduodenal pathologies in the Cukurova region of Turkey. J. Clin. Microbiol., 47: 4150-4153.

Pereira, PM and Vicente, AF (2013). Meat nutritional composition and nutritive role in the human diet. Meat Sci., 93: 586-592.

Quaglia, N; Dambrosio, A; Normanno, G; Parisi, A; Patrono, R; Ranieri, G; Rella, A and Celano, G (2008). High occurrence of Helicobacter pylori in raw goat, sheep and cow milk inferred by glmM gene: a risk of food-borne infection? Int. J. Food. Microbiol., 124: 43-47.

Rahimi, E and Kheirabadi, EK (2012). Detection of Helicobacter pylori in bovine, buffalo, camel, ovine, and caprine milk in Iran. Foodborne. Pathog. Dis., 9: 453-456.

Saeidi, E and Sheikhshahrokh, A (2016). VacA genotype status of Helicobacter pylori isolated from foods with animal origin. Biomed. Res. Int., 2016: 1-6.

Talebi Bezmin Abadi, A; Mobarez, AM; Taghvaei, T and Wolfram, L (2010). Antibiotic resistance of Helicobacter pylori in Mazandaran, north of Iran. Helicobacter. 15: 505-509.

Valsta, L; Tapanainen, H and Männistö, S (2005). Meat fats in nutrition. Meat Sci., 70: 525-530.

Van Duynhoven, YT and Jonge, RD (2001). Transmission of Helicobacter pylori: a role for food? Bull. World Health Org., 79: 455-460.

Van Leerdam, M and Tytgat, G (2002). Helicobacter pylori infection in peptic ulcer haemorrhage. Aliment. Pharmacol. Ther., 16(s1): 66-78.

Wayne, P (2012). Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial suscep-tibility testing. Twenty-second informational supplement M100-S21. United States.

Webberley, MJ; Webberley, JM; Newell, DG; Lowe, P and Melikian, V (1993). Seroepidemiology of Helicobacter pylori infection in vegans and meat-eaters. Epidemiol. Infect., 108: 457-462.

Yahaghi, E; Khamesipour, F; Mashayekhi, F; Safarpoor Dehkordi, F; Sakhaei, MH; Masoudimanesh, M and Khameneie, MK (2014). Helicobacter pylori in vegetables and salads: genotyping and antimicrobial resistance properties. Biomed. Res. Int., Article ID 75794.

Yamada, R; Yamaguchi, A and Shibasaki, K (2008). Detection and analysis of Helicobacter pylori DNA in the gastric juice, saliva, and urine by nested PCR. Oral. Sci. Int., 5: 24-34.