تاثیر FSH و eCG بر روی تکامل فولیکول‌ها و بیان کاسپاز-9 و FSHR تخمدان در موش سوری

نوع مقاله : مقاله کامل

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چکیده

هدف مطالعه حاضر ارزیابی اثرات FSH و eCG بر روی تکامل تخمدان و فولیکول، سطوح بیان FSHR و کاسپاز-9 تخمدان‌ها در محیط داخل بدن بود. تعداد 105 موش سوری پیش از بلوغ به گروه‌های FSH-1، FSH-2، FSH-3، eCG-1، eCG-2، eCG-3 و گروه کنترل (CG) اختصاص داده شدند. به موش‌ها در گروه‌های FSH-1، FSH-2 و FSH-3 به صورت داخل عضلانی دو بار (روز 0 و 4) به ترتیب با 5، 10 و 20 IU FSH تزریق شدند. موش‌ها در گروه‌های eCG-1، eCG-2 و eCG-3 در روز 0 و 4 به صورت داخل صفاقی با 10، 20 و 40 IU eCG مورد تزریق قرار گرفتند. به موش‌ها در گروه کنترل 5/0 میلی لیتر نرمال سالین در روز 0 و 4 تزریق شد. تخمدان‌های چپ و راست هر موش به صورت استریل به ترتیب در روزهای 7، 14 و 21 تشریح شدند. نتایج نشان دادند که در روز 14 و 21 اندازه تخمدان‌ها و تعداد فولیکول‌های گروه‌های FSH-3 و eCG-3 بیشتر از CG بودند (P<0.05). FSHR mRNA در گروه‌های FSH-2 و eCG-1 در روزهای 14 و 21 بیشتر از CG بودند (P<0.05). پروتین‌های FSHR گروه FSH-3 در روزهای 14 و 21 بیشتر از CG بودند (P<0.05). mRNA کاسپاز-9 در گروه‌های FSH و eCG کمتر از CG بودند. روابط مثبتی بین تعداد فولیکول‌ها و دوزهای FSH و eCG وجود داشت. بیان پروتئین FSHR ارتباط مثبتی بین وزن و اندازه تخمدان و تعداد فولیکول‌ها در موش‌های درمان شده با FSH داشت (r=0.971, P<0.05). غلظت سرمی FSH در گروه‌های FSH-2، FSH-3، eCG-2 و eCG-3 بیشتر از آن در گروه CG بود. در نتیجه، eCG و FSH تکامل تخمدانی، تشکیل فولیکول، ترشح FSH، بیان mRNA و پروتئین FSHR را در تخمدان‌های موش‌های سوری افزایش دادند. FSH و eCG بیان mRNA کاسپاز-9 تخمدان را مهار کردند.

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