Determination of renal handling of marbofloxacin in Lohi sheep (Ovis aries) following a single intravenous administration

Document Type: Full paper (Original article)

Authors

Department of Pharmacology, Al-Nafees Medical College, Isra University, Islamabad, Pakistan

Abstract

The objective of present study was to investigate renal clearance, urinary excretion and underlying excretory mechanism of marbofloxacin in Lohi sheep. For this purpose, marbofloxacin was administered intravenously (IV) as single bolus dose (2.5 mg/kg body weight) to eight healthy sheep of Lohi breed. After start of experiment, blood and urine samples were drawn at predetermined time intervals and marbofloxacin concentrations in the samples were measured by reverse phase high performance liquid chromatography (RP-HPLC) using UV/Vis detector. The mean ± SD values of creatinine in plasma and urine were 15.37 ± 0.65 µg/ml and 246.7 ± 48.05 µg/ml, respectively. Glomerular filtration rate was 1.29 ± 0.22 ml/min/kg whereas urinary flow rate was observed to be 0.084 ± 0.016 ml/min/kg. The renal clearance of marbofloxacin in Lohi sheep was 9.45 ± 2.12 ml/min/kg. Cumulative percentage dose excreted was seen to be maximum at 24 h post drug administration. It was concluded that renal handling of marbofloxacin in Lohi sheep involved both glomerular filtration and active tubular secretion.

Keywords


Afzal, G; Nawaz, M and Saleem, B (1982). Renal clearance of sulphadimidine in sheep. Pak. Vet. J., 2: 57-60.

Alvi, MA; Hashmi, AS and Nawaz, M (1985). Renal clearance of exogenous and endogenous urea in sheep. Pak. Vet. J., 5: 133-136.

Blum, RA (1992). Influence of renal function on the pharma-cokinetics of lomefloxacin compared with other fluoro-quinolones. Am. J. Med., 92: 18S-21S.

Christopher, JL (2012). Principles of renal physiology. (5th Edn.), USA, Springer Publishing. PP: 37-39.

Garcia, MA; Solans, C; Aramayona, JJ; Rueda, S and Bregante, MA (1999). Determination of marbofloxacin in plasma samples by high-performance liquid chromato-graphy using fluorescence detection. J. Chromatogr. b., 729: 157-161.

Giles, CJ; Magonigle, RA; Grimshaw, WTR; Tanner, AC; Risk, JE; Lynch, MJ and Rice, R (1991). Clinical pharmacokinetics of parenterally administered danofloxa-cin in cattle. J. Vet. Pharmacol. Ther., 14: 400-410.

Iqbal, Z; Javed, I; Basit, A; Jan, I and Khan, AA (2011). Pharmacokinetics and dosage regimen of ciprofloxacin following single intramuscular administration in Teddy goats. Pak. J. Pharm. Sci., 24: 69-74.

Iqbal, Z; Khan, AA; Hasnain, J and Javed, I (2012). Renal clearance and urinary excretion of ciprofloxacin in Lohi sheep. Pak. J. Pharmacol., 29: 39-44.

Iqbal, Z; Khan, AA; Jan, I; Mehmood, MA and Javed, I (2015). Pharmacokinetics and dosage regimen of cip-rofloxacin following single intramuscular administration in Nili/Ravi buffalos. Asian J. Agri. Biol., 3: 28-33.

Javed, I; Iqbal, Z; Rahman, ZU; Khan, MZ; Muhammad, F; Aslam, B; Sandhu, MA and Sultan, JI (2009). Disposition kinetics and optimal dosage of ciprofloxacin in healthy domestic ruminant species. Acta Vet. Burno. 78: 155-162.

Javed, I; Khan, FH; Muhammad, F; Aslam, B; Khaliq, T; Iqbal, Z; Ali, L and Mujib, S (2005). Renal clearance and urinary excretion of norfloxacin in sheep. Pak. Vet. J., 25: 51-54.

Javed, I; Nawaz, M and Khan, FH (2003). Pharmacokinetics and optimal dosage of kanamycin in domestic ruminant species. Vet. Arhiv., 73: 323-331.

Karademir, U; Boyacioglu, M; Kum, C and Sekkin, S (2015). Comparative pharmacokinetics of enrofloxacin, danofloxacin and marbofloxacin following intramuscular administration in sheep. Small Rumin. Res., 133: 108-111.

Kunin, CM; Dornbus, RJ and Finland, M (1959). Distribution and excretion of tetracycline analogues in normal young man. J. Clin. Invest., 38: 1950-1963.

Manzoor, Z and Iqbal, Z (2016). Pharmacokinetics and dosage regimen of danofloxacin by RP-HPLC/UV method in Teddy goats. Lat. Am. J. Pharm., 35: 172-179.

Muhammad, F; Hussain, F; Nawaz, M and Javed, I (2003). Disposition kinetics of kanamycin in mules. Vet. Arhiv., 73: 221-226.

Nawaz, M; Ilahi, A and Iqbal, Z (1992). Metabolism of 2-(P-amino benzen sulphonamide)-pyrimidine (sulphadiazine) in buffaloes. Pak. Vet. J., 12: 116-120.

Ram, D; Dumka, VK; Sharma, SK and Sandhu, SH (2008). Pharmacokinetics, dosage regimen and in vitro plasma protein binding of intramuscular levofloxacin in buffalo
calves. Iran. J. Vet. Res., 9: 121-126.

Sidhu, PK; Landoni, MF; Aliabadi, FS and Lees, P (2010). PK-PD integration and modeling of marbofloxacin in sheep. Res. Vet. Sci., 88: 134-141.

Swenson, MJ (1985). Duke’s physiology of domestic animals. (9th Edn.), USA, Comstock Publishing Associates. PP: 443-492.