Echocardiographic assessment of myocardial infarction: comparison of a rat model in two strains

Document Type: Full paper (Original article)

Authors

1 Ph.D. Student in Genetics, Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Cancer Genetics Department, Breast Cancer Research Center (BCRC), (ACECR), Tehran, Iran

2 Echocardiography Research Center, Rajaie Cardiovascular, Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran

3 DVM, Rajaie Cardiovascular, Medical and Research Centre, Iran University of Medical Sciences, Tehran, Iran

4 Cancer Genetics Department, Breast Cancer Research Center (BCRC), (ACECR), Tehran, Iran; Tasnim Biotechnology Research Center (TBRC), Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran

5 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

6 Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

The purpose of this study was to induce myocardial infarction (MI) and compare the echocardiographic parameters and mortality ratio of Lewis inbred and Wistar outbred strain before and after the procedure to help choose the best one for MI studies. In this study MI was induced in 46 Lewis and 34 Wistar by occlusion of left anterior descending artery (LAD). Doppler, two-dimensional (2-D) and 2-D guided M-mode images were recorded from parasternal long-axis and parasternal short-axis and apical four-chamber views. The following parameters were acquired. Interventricular septum diastolic and systolic dimension (IVSd, s), diastolic and systolic left ventricular internal diameter (LVIDd, s), diastolic and systolic left ventricular posterior wall dimension (LVPWd, s), ejection fraction (EF), and fractional shortening (FS). The significant changes were observed in systolic IVS, LVID and EF and FS before and after MI and no significant difference was detected between Lewis and Wistar. The high mortality rate of 51% was seen in the procedure, including anesthesia in Lewis compared to 34% in Wistar. As a conclusion the echocardiographic parameters of these two strains were similar, but according to mortality rate and more cardiac anatomic variation in Lewis rats, Wistar is better for MI studies.

Keywords


Avsaroglu, H; Sommer, R; Hellebrekers, LJ; Van Zutphen, LF and Van Lith, HA (2008). The effects of buprenor-phine on behaviour in the ACI and BN rat inbred strains. Lab. Anim., 42: 171-184.

Avsaroglu, H; Van Der Sar, AS; Van Lith, HA; Van Zutphen, LF and Hellebrekers, LJ (2007). Differences in response to anaesthetics and analgesics between inbred rat strains. Lab. Anim., 41: 337-344.

Cittadini, A; Strömer, H; Katz, SE; Clark, R; Moses, AC; Morgan, JP and Douglas, PS (1996). Differential cardiac effects of growth hormone and insulin-like growth factor1 in the rat A combined in vivo and in vitro evaluation. Circulation. 93: 800-809.

Darbandi Azar, A; Tavakoli, F; Moladoust, H; Zare, A and Sadeghpour, A (2014). Echocardiographic evaluation of cardiac function in ischemic rats: value of M-mode echocardiography. Res. Cardiovasc. Med., 3: e22941.

Henke, J; Astner, S; Brill, T; Eissner, B; Busch, R and Erhardt, W (2005). Comparative study of three intra-muscular anaesthetic combinations (medetomidine/ ketamine, medetomidine/fentanyl/midazolam and xylazine/ ketamine) in rabbits. Vet. Anaesth. Analg., 32: 261-270.

Holinski, S; Knebel, F; Heinze, G; Konertz, W; Baumann, G and Borges, AC (2011). Noninvasive monitoring of cardiac function in a chronic ischemic heart failure model in the rat: assessment with tissue Doppler and non-Doppler 2D strain echocardiography. Cardiovasc. Ultrasound., 9: 15-21.

Jin, J; Jeong, SI; Shin, YM; Lim, KS; Lee, YM; Koh, HC and Kim, KS (2009). Transplantation of mesenchymal stem cells within a poly (lactide-co-epsilon-caprolactone) scaffold improves cardiac function in a rat myocardial infarction model. Eur. J. Heart Fail., 11: 147-153.

Klocke, R; Tian, W; Kuhlmann, MT and Nikol, S (2007). Surgical animal models of heart failure related to coronary heart disease. Cardiovasc. Res., 74: 29-38.

Kusunose, K; Penn, MS; Zhang, Y; Cheng, Y; Thomas, JD; Marwick, TH and Popović, ZB (2012). How similar are the mice to men? Between-species comparison of left ventricular mechanics using strain imaging. PLoS ONE. 7: e40061.

Lee, WY; Wei, HJ; Wang, JJ; Lin, KJ; Lin, WW; Chen, DY; Huang, CC; Lee, TY; Ma, HY; Hwang, SM; Chang, Y and Sung, HW (2012). Vascularization and restoration of heart function in rat myocardial infarction using transplantation of human cbMSC/HUVEC core-shell bodies. Biomaterials. 33: 2127-2136.

Leenen, FH and Yuan, B (2001). Mortality after coronary artery occlusion in different models of cardiac hypertrophy in rats. Hypertension. 37: 209-215.

Liao, SS; Ruan, QY; Lin, MY and Yan, L (2012). Value of segmental myocardial strain by 2-dimensional strain echocardiography for assessment of scar area induced in a rat model of myocardial infarction. Cardiovasc. Ultrasound., 10: 17-25.

Liu, Y; Asnani, A; Zou, L; Bentley, VL; Yu, M; Wang, Y; Dellaire, G; Sarkar, KS; Dai, M; Chen, HH; Sosnovik, DE; Shin, JT; Haber, DA; Berman, JN; Chao, W and Peterson, RT (2014). Visnagin protects against doxorubicin-induced cardiomyopathy through modulation of mitochondrial malate dehydrogenase. Sci. Transl. Med., 6: 266-270.

Liu, YH; Yang, XP; Nass, O; Sabbah, HN; Peterson, E and Carretero, OA (1997). Chronic heart failure induced by coronary artery ligation in Lewis inbred rats. Am. J. Physiol., 272: 722-727.

Matsumoto, R; Omura, T; Yoshiyama, M; Hayashi, T; Inamoto, S; Koh, KR; Ohta, K; Izumi, Y; Nakamura, Y; Akioka, K; Kitaura, Y; Takeuchi, K and Yoshikawa, J (2005). Vascular endothelial growth factor-expressing mesenchymal stem cell transplantation for the treatment of acute myocardial infarction. Arterioscler. Thromb. Vasc. Biol., 25: 1168-1173.

Meagher, S; Penn, DJ and Potts, WK (2000). Male-male competition magnifies inbreeding depression in wild house mice. Proceedings of the National Academy of Sciences. 97: 3324-3329.

Pawlush, DG; Moore, RL; Musch, TI and Davidson, WR (1993). Echocardiographic evaluation of size, function, and mass of normal and hypertrophied rat ventricles. J. Appl. Physiol., 74: 2598-2605.

Pieper, GM; Shah, A; Harmann, L; Cooley, BC; Ionova, IA and Migrino, RQ (2010). Speckle-tracking 2-dimensional strain echocardiography: a new noninvasive imaging tool to evaluate acute rejection in cardiac transplantation. J. Heart Lung Transplant., 29: 1039-1046.

Scheer, P; Sverakova, V; Doubek, J; Janeckova, K; Uhrikova, I and Svoboda, P (2012). Basic values of M-mode echocardiographic parameters of the left ventricle in outbreed Wistar rats. Vet. Med. (Praha)., 57: 42-52.

Stein, JH; Neumann, A; Preston, LM; Costanzo, MR; Parrillo, JE; Johnson, MR and Marcus, RH (1997). Echocardiography for hemodynamic assessment of patients with advanced heart failure and potential heart transplant recipients. J. Am. Coll. Cardiol., 30: 1765-1772.

Sun, L; Cui, M; Wang, Z; Feng, X; Mao, J; Chen, P; Kangtao, M; Chen, F and Zhou, C (2007). Mesenchymal stem cells modified with angiopoietin-1 improve remodeling in a rat model of acute myocardial infarction. Biochem. Biophys. Res. Commun., 357: 779-784.

Szmit, S; Streb, J; Starzec, W; Zuziak, D; Kwiatkowski, M; Czartoryska-Arłukowicz, B; Iżycki, D; Śmiałowska-Janiszewska, A; Hanslik, J; Bryjak, A; Talerczyk, M and Zaucha, JM (2015). Left ventricular systolic dysfunction in metastatic breast cancer patients: a polish multicenter registry. Anticancer Res., 35: 989-995.

Tavakoli, F; Ostad, SN; Khori, V; Alizadeh, AM; Sadeghpour, A; Darbandi Azar, A; Haghjoo, M; Zare, A and Nayebpour, M (2013). Outcome improvement of cellular cardiomyoplasty using triple therapy: mesenchymal stem cell+erythropoietin+vascular endothelial growth factor. Eur. J. Pharmacol., 714: 456-463.

Van Den Borne, SWM; Van De Schans, VA; Strzelecka, AE; Vervoort-Peters, HTM; Lijnen, PM; Cleutjens, JPM; Smits, JFM; Daemen, MJ; Janssen, BJA and Blankesteijn, WM (2009). Mouse strain determines the outcome of wound healing after myocardial infarction. Cardiovasc. Res., 84: 273-282.

Watson, LE; Sheth, M; Denyer, RF and Dostal, DE (2004). Baseline echocardiographic values for adult male rats. J. Am. Soc. Echocardiogr., 17: 161-167.

White, HD and Chew, DP (2008). Acute myocardial infarction. Lancet. 372: 570-584.

Wixson, S; White, W; Hughes HC, Jr; Lang, C and Marshall, W (1987). The effects of pentobarbital, fentanyl-droperidol, ketamine-xylazine and ketamine-diazepam on core and surface body temperature regulation in adult male
rats. Lab. Anim. Sci., 37: 743-749.

Wollert, KC; Studer, R; Von Bulow, B and Drexler, H (1994). Survival after myocardial infarction in the rat. role of tissue angiotensin-converting enzyme inhibition. Circulation. 90: 2457-2467.

Wu, Y; Yin, X; Wijaya, C; Huang, MH and Mcconnell, BK (2011). Acute myocardial infarction in rats. J. Vis. Exp., 24: 1-4.

Zerna, C; Guenther, M; Folprecht, G and Puetz, V (2015). Acute ischaemic stroke and myocardial infarction after chemotherapy with vinorelbine for non-small cell lung cancer: a case report. J. Chemother., 197: 394-403.

Zornoff, LA; Paiva, SA; Minicucci, MF and Spadaro, J (2009). Experimental myocardium infarction in rats: analysis of the model. Arq. Bras. Cardiol., 93: 426-432, 434-440.