Effects of dietary supplementation of mannan-oligosaccharide on virus shedding in avian influenza (H9N2) challenged broilers

Document Type: Short paper


1 MPhil/Ms in Physiology, Department of Physiology, University of Veterinary and Animal Sciences, Lahore-5400, Punjab, Pakistan

2 MPhil/Ms in Genetic Engineering and Biotechnology, Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar-25120, KPK, Pakistan

3 MPhil/Ms in Livestock Management, Department of Livestock Management, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar-25120, KPK, Pakistan

4 MPhil/Ms in Biotechnology, Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

5 Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar-25120, KPK, Pakistan


Avian influenza (AI) is a highly contagious disease causing significant economic losses worldwide. The aim of this study is to evaluate the effect of mannan-oligosaccharide (MOS) on tracheal and cloacal virus shedding in AI challenged broilers and contamination of environment with H9N2. A total of 300 1-day-old-broiler chicks were randomly divided into 3 groups (A, B and C) and supplemented 0.2, 0.5 and 0.0% MOS, respectively in NRC recommended diet for 36 days. On day 21 the groups were further split into two sub groups A+ve, A-ve, B+ve, B-ve, C+ve and C-ve with 5 replicates each. The positive groups were shifted to remote sheds and were challenged intranasally with 0.1 ml of reference virus (AIV; Pk-UDL/01/08 H9N2) with EID50 = 10-6.66. Treatment reduces (P<0.05) cloacal virus shedding from day 24 to 26 and 28 to 32. Tracheal virus shedding was lower (P<0.05) on days 25-26 and 28-30 in treatment groups. Day 27 showed highest (P>0.05) virus shedding in all groups. However the reduction of viral shedding is faster in treatment groups and showed no virus shedding on day 32. Maternal antibody titer against AI showed a declining pattern but MOS influenced (P<0.05) the titer in treated groups. Hence the use of MOS may constitute a novel and effective plausible alternative that reduces the spread of disease by decreasing virus shedding and contamination of environment from AIV (H9N2) infection in poultry.


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