1Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
2Department of Veterinary Obstetrics and Gynecology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
3Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO), catalase (CAT), super oxide dismutase (SOD) activity, and total protein (TP) were estimated in seminal plasma. The protein profiling was carried out by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The significant effect of season was observed on TP, SOD activity and % protein fractions of seminal plasma proteins of buffalo bulls. The TP values showed positive correlation with ejaculate volume (EV), sperm concentration (SC), and % live-dead (LD) and negative correlation with progressive motility (PM), and hypo-osmotic swelling test (HOST). The SOD activity showed positive correlation with PM, LD, HOST and % acrosoamal integrity (AI). Besides that, results showed correlation of TP with 6.5, 38 and 66 kDa proteins, LPO with 70, 72, 84 and 86 kDa proteins, CAT with 70 kDa and 86 kDa proteins, and SOD with 6.5, 24.5, 44.5, 70 and 72 kDa proteins. In conclusion, this study indicated that TP and SOD activity of seminal plasma of buffalo bulls were influenced by season and were found to be associated with some of the semen characteristics and expression of seminal plasma proteins.
Agarwal, A and Saleh, RA (2002). Role of oxidants in male infertility: rationale, significance, and treatment. Urol. Clin. North Am., 29: 817-827.
Alvarez, JG and Storey, BT (1989). Role of glutathione-peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid-peroxidation. Gamete Res., 23: 77-90.
Atig, F; Raffa, M; Ali, HB; Abdel-hamid, K; Saad, A and Ajina, M (2012). Altered antioxidant status and increased lipid per-oxidation in seminal plasma of Tunisian infertile men. Int. J. Biol. Sci., 8: 139-149.
Baumber, J; Ball, BA; Gravance, CG; Medina, V and Davies-Morela, MCG (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential and membrane lipid peroxidation. J. Androl., 21: 895-902.
Bergmeyer, HU; Grassl, M and Walter, HE (1983). Catalase. In: Bergmeyer, HU (Ed.), Methods of enzymatic analysis. (3rd Edn.), Vol. 2, Verlag Chemie, Weinheim. PP: 165-166.
Berry, EM and Kohen, R (1999). Is the biological antioxidant system integrated and regulated? Med. Hypoth., 53: 397-401.
Cardozo, A; Fernandez, M; Forcada, F; Abecia, A; Muino-Blanco, T and Cebrian-Perez, A (2006). Monthly varia-tion in ovine seminal plasma proteins analyzed by 2D SDS-PAGE. Theriogenology. 66: 841-850.
Chen, S; Chang, L and Wei, Y (2001). Oxidative damage to proteins and decrease of antioxidant capacity in patients with varicocele. Free Radic. Biol. Med., 30: 1328-1334.
de Souza, FF; Barreto, CS and Lopes, MD (2007). Characte-ristics of seminal plasma proteins and their correlation with canine semen analysis. Theriogenology. 68: 100-106.
Hammadeh, ME; Filippos, A and Hamad, MF (2009). Reactive oxygen species and antioxidant in seminal plasma and their impact on male fertility. Int. J. Fertil. Steril., 3: 87-110.
Hampl, R; Drabkova, P; Kandar, R and Stepan, J (2012). Impact of oxidative stress on male infertility. Ceska Gynekol., 77: 241-245.
Hsieh, YY; Sun, YL; Chang, CC; Lee, YS; Tsai, HD and Lin, CS (2002). Super oxide dismutase activities of spermatozoa and seminal plasma are not correlated with male infertility. J. Clin. Lab. Anal., 16: 127-131.
Jimenez, C; Ghyselinck, NB; Depeiges, A and Dufaure, JP (1990). Immunochemical localization and association with spermatozoa of androgen-regulated proteins of mr-24000 secreted by the mouse epididymis. Biol. Cell., 68: 171-174.
Kadirvel, G; Kumar, SK; Ghosh, SK and Perumal, P (2014). Activity of antioxidative enzymes in fresh and frozen thawed buffalo (Bubalus bubalis) spermatozoa in relation to lipid peroxidation and semen quality. Asian Pacif. J. Reprod., 3: 210-217.
Khawaskar, M; Panchal, MT; Dhami, AJ; Hadiya, KK and Patel, SB (2012). Seasonal variation in seminal bio-chemical constituents in Surti buffalo bulls. Ind. J. Anim. Reprod., 33: 41-46.
Kowalowka, M; Wysocki, P; Fraser, L and Strzezek, J (2007). Extracellular super oxide dismutase of boar seminal plasma. Reprod. Domes. Anim., 44: 490-496.
Kumar, S; Tripathi, SS and Saxena, VB (1984). A com-parative study on phosphatase, sodium and potassium in successive semen ejaculates of Red Dane, Jersey and Murrah bulls. Cheiron. 13: 136-139.
Laemelli, UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 220: 680-685.
Lenzi, A; Gandini, L; Maresca, V; Rago, R; Sgro, P; Dondero, F and Picardo, M (2000). Fatty acid com-position of spermatozoa and immature germ cells. Mol. Hum. Reprod., 6: 226-231.
Madesh, M and Balasubramanian, KA (1998). Microtiter plate assay for super-oxide dismutase using MTT reduction by super-oxide. Ind. J. Biochem. Biophy., 35: 184-188.
Mandal, DK; Nagpaul, PK and Gupta, AK (2000). Seasonal variation in seminal attributes and sexual behaviour of Murrah buffalo bulls. Ind. J. Dairy Sci., 53: 278-283.
Marti, E; Mara, L; Marti, JI; Muino-Blanco, T and Cebrian-Perez, JA (2007). Seasonal variations in anti-oxidant enzyme activity in ram seminal plasma. Theriogenology. 67: 1446-1454.
Nandre, RM (2008). Effect of preservation of spermatozoa at sub-zero temperature on DNA integrity by comet assay. M.V.Sc Thesis, Submitted to Anand Agricultural University. P: 63.
Neild, DM; Brouwers, JF; Colenbrander, B; Aguero, A and Gadella, BM (2005). Lipid peroxide formation in relation to membrane stability of fresh and frozen thawed stallion spermatozoa. Mol. Reprod. Dev., 72: 230-238.
Nichi, M; Bols, PEJ; Zuge, RM; Barnabe, VH; Goovaerts, IGF; Barnabe, RC and Cortada, CNM (2006). Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenololgy. 66: 822-828.
Pandey, V; Nigam, R; Saxena, A; Singh, P; Sharma, A; Swain, DK and Sharma, L (2014a). Seasonal variations in biochemical attributes of Hariyana bull semen. Rumi. Sci., 3: 19-24.
Pandey, V; Nigam, R; Saxena, A; Singh, P; Sharma, A; Swain, DK; Sharma, L and Dixit, S (2014b). Influence of season on biochemical attributes of Bhadawari buffalo bull semen: effect of temperature and humidity. J. Anim. Res., 4: 201-209.
Rehman, SU (1984). Lead induced regional lipid per-oxidation in brain. Toxicol. Lett., 21: 333-337.
Saleh, RA and Agarwal, A (2002). Oxidative stress and male infertility: from research bench to clinical practice. J. Androl., 23: 737-752.
Selvaraju, S; Siva-Subramani, T; Raghavendra, BS and Ravindra, JP (2010). Effect of IGF-I on spermatozoa membrane protein profile and correlation between seminal plasma IGF-I and antioxidant enzymes in buffalo semen. Ind. J. Anim. Sci., 80: 1171-1174.
Shamsi, MB; Venkatesh, S; Tanwar, M; Talwar, P; Sharma, RK; Dhawan, A; Kumar, R; Gupta, NP; Malhotra, N; Singh, N; Mittal, S and Dadaa, R (2009). DNA integrity and semen quality in men with low seminal antioxidant levels. Mutat. Res., 665: 29-36.
Sharma, L; Pandey, V; Nigam, R; Singh, P; Saxena, A and Swain, DK (2014). Seasonal variations in seminal plasma proteins of buffalo. Reprod. Dom. Anim., 49: 387-391.