1Department of Genetic, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran
2Department of Biology, School of Biological Sciences, University Science Malaysia (USM), Penang, Malaysia
3Department of Biotechnology, School of Agricultural Sciences, Bu-Alisina University, Hamadan, Iran
4Department of Biology, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran
5Young Researchers and Elite Club, Bandar Abass Branch, Islamic Azad University, Bandar Abass, Iran
In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship of Trichiurus lepturus in the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs to T. lepturus showing 42 divergence sites among the same reported partial sequences of 16S rRNA gene from the other area (West Atlantic and Indo-Pacific area). Phylogeny results showed that all 18 haplotypes of the species clustered into five clades with reasonably high bootstrap support of values (>64%). Overall, the tree topology for both phylogenetic and phenetic trees for 16S rDNA was similar. Both trees exposed two major clusters, one wholly containing the haplotypes of the T. lepturus species belonging to Indo-Pacific area with two major sister groups including Persian Gulf specimen and the other cleared the Western Atlantic and Japan individuals clustered in another distinct clade supporting the differentiation between the two areas. Phylogenic relationship observed between the Persian Gulf and the other Indo-Pacific Individuals suggested homogeneity between two mentioned areas.
Allard, MW; Miyamoto, MM; Jareche, L; Kraus, F and Tennant, MR (1992). DNA systematics and evolution of the artiodactyls family Bovidae. Proc. Natl. Acad. Sci. USA., 89: 3972-3976.
Baik, CI and Park, JH (1986). Relationship between oceanographic condition and catch of the hairtail, Trichiurus lepturus Linn C from the Stow Net. Bull. Fish Res. Dev. Agency. 39: 29-41.
Bourdy, P; Heurtebise, S and Lapegue, S (2003). Mitochondrial and nuclear DNA sequence variation of presumed Crassostrea gigas and Crassostrea angulata specimens: a new oyster species in Hong Kong. Aquaculture. 228: 15-25.
Brown, WM; Prager, EM; Wang, A and Wilson, AC (1982). Mitochondrial DNA sequences of primates: tempo and mode of evolution. J. Mol. Evol., 18: 225-239.
Dekun, Z and Cungen, Y (1987). The relation of the environment of fishing ground with the occurrence of hairtail in winter off the middle part of Zhejiang. 1. Fish. China. 11: 195-204.
Duke, NC (1992). Mangrove floristics and biogeography. PP: 63-100. In: Robertson, AI and Alongi, DM (Eds.), Tropical mangrove ecosystems, coastal and estuarine studies series. American Geophysical Union, Washington, D.C., P: 329.
Hall, BG (2004). Phylogenetics trees made easy: a how-to manual for molecular biologists, Sunderland, Massa-chusetts, Sinauer Associates. P: 443.
Kimura, M (1980). A simple method for estimating evolu-tionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 16: 111-120.
Kimura, S and Matsuura, K (2003). Fishes of Bitung, northern tip of Sulawesi, Indonesia. Tokyo, Ocean Research Institute, University of Tokyo. P: vi+244.
Lam, K and Morton, B (2003). Mitochondrial DNA and morphological identification of a new species of Crassostrea (Bivalvia: Ostreidae) cultured for centuries in the Pearl River Delta, Hong Kong, China. Aquaculture. 228: 1-13.
Martins, AS and Haimovici, M (1996). Distribution, abundance and biological interactions of the cutlassfish Trichiurus lepturus in the southern Brazil subtropical convergence ecosystem. Fish. Res., 30: 217-227.
Meriem, SB; Al-Marzouqi, A; Al-Mamry, J and Al-Mazroui, A (2011). Stock assessment and potential management of Trichiurus lepturus fisheries in the Arabian Sea, Oman. J. Fish Aquat. Sci., 6: 212-224.
Milinkovitch, MC; Orti, G and Meyer, A (1993). Revised phylogeny of whates suggested by mitochondrial ribosomal DNA sequences. Nature. 361: 346-348.
Miya, M and Nishida, M (1996). Molecular phylogenetic perspective on the evolution of the deep-sea fish genus Cyclothone (Stomiiformes: Gonostomatidae). Ichthyol. Res., 43: 375-398.
Nakabo, T (2002). Family Trichiuridae. In: Nakabo, T (Ed.), Fishes of Japan with pictorial keys to the species. Tokyo, TokaiUniversity Press. PP: 1344-1345.
Nakamura, I and Parin, NV (1993). FAO species catalogue. Vol. 15. Snake mackerels and cutlassfishes of the world (Families Gempylidae and Trichiuridae). An annotated andillustrated catalogue of the snake mackerels, shocks, escolars, gemfishes, sack fishes, domine, oilfish, cutlassfishes, scabbardfishes, hair-tails, and frostfishes known todate. Fisheries Synopsis No. 125, Vol. 15, FAO, Rome, P: 136.
Raeisi, H; Hosseini, SA; Paighambari, SY; Taghavi, SAA and Davoodi, R (2011). Species composition and depth variation of cutlassfish (Trichiurus lepturus L. 1785) trawl by catch in the fishing grounds of Bushehr waters. Persian Gulf. 10: 17610-17619.
Randall, JE (1995). Coastal fishes of Oman. Bathurst, New South Wales, Australia, Crawford House Publishing Pty Ltd., P: 439.
Reynolds, RM (1993). Physical oceanography of the Gulf, Strait of Hormuz, and the Gulf of Oman: results from the Mitchell Expedition. Marine Poll. Bull., 27: 35-60.
Sambrook, J; Fritsch, EF and Maniatis, T (1989). Molecular cloning: a laboratory manual. 2nd Edn., Cold Spring Harbor, NY, ColdSpringHarbor Laboratory Press. P: 1622.
Tamura, K; Dudley, J; Nei, M and Kumar, S (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA software). Version 4.0. Mol. Biol. Evol., 24: 1596-1599.
Tokimura, M; Yamada, U and Irie, T (1995). Comments on taxonomy and distributions of the species of Trichiurus in the East China and YellowSeas and adjacent waters. Seikaku Suisan Kenkyusho News. 80: 12-14.
UNEP (United Nations Environment Programme) (1999). Overview on land-based sources and activities affecting the marine environment in the ROPME Sea area. UNEP/GPA Coordination Office & ROPME. P: 127.
Yamada, U; Shirai, S; Irie, T; Tokimura, M; Deng, S; Zheng, Y; Li, CS; Kim, YU and Kim, YS (1995). Names and illustrations of fishes from the East China Sea and the Yellow Sea. Tokyo, Overseas Fishery Cooperation Foundation. P: xi+288.