1Department of Parasitology, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran
2Department of Parasitology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Department of Microbiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5Department of Mathematic and Statistics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
To develop an ELISA technique for the detection of antibodies against Babesia ovis, the infected erythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of the lysate was used as antigen. Optimal dilution of the antigen was determined by checkerboard titrations, using positive and negative control sera. A correlation of 85% was observed between the results of the developed ELISA and IFA techniques. To study the seroprevalence of Babesia ovis in Khouzestan province, south of Iran, a total of 1000 sheep sera were collected from different areas of the province and tested against Babesia ovis using the ELISA technique developed. The results showed an average seroprevalence of 47.5% in the province. Our results indicated a significant increase of the seroprevalence by advancement of age of the animals. There was no significant difference between the seroprevalence of female and male sheep.