Comparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens

Document Type: Short paper

Authors

1 Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Clinical Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Graduated from School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsuming
procedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94
different specimens taken from animal origin were cultured on brucella selective culture media and colonies
were identified biochemically. The specimens were also examined after centrifugation by Dot-ELISA using a
specific anti-brucella antibody, a suitable peroxidase conjugate and substrate. Of the 94 samples, 5 (5.31%)
were positive in Dot-ELISA and 4 (4.25%) had positive cultures. In comparison with culture, the sensitivity
and specificity of Dot-ELISA for detection of brucellae in the samples was 80 and 100%, respectively. There
was 98.9% agreement between the two tests. The results indicated that Dot-ELISA is a good and rapid test
with acceptable sensitivity and specificity for detection of Brucella spp. in aborted fetal stomach contents.

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