Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran

Document Type: Full paper (Original article)

Authors

1 Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran

2 Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karadj, Iran

3 1Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran

Abstract

Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule has
been implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction
(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified a
fragment of 1044 bp from all of tested isolates. It was found that all avian P. multocida isolates belonged to
capsular type A. The sequence alignment of the fragment showed a high similarity (>96%) with the
published sequences of P. multocida hya gene in the Gene Bank. It was recognised that P. multocida capsular
group A is the dominant cause of fowl cholera in Iran.

Keywords