Molecular detection of Leptospira spp. in the urine of cattle in northern Iran

Document Type: Short paper

Authors

1 Department of Microbiology and Genetics, College of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran

2 Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Department of Veterinary Medicine, College of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Iran

5 Member of the Iranian Epidemiological Association (IrEA), Tehran, Iran

Abstract

Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans and is considered as an emerging global public health problem. Transmission usually results from direct or indirect exposure to the urine or other body fluids of leptospiruric animals which may become a source of infection for human or other animals. Having a humid climate with plenty of annual rainfall, Guilan province is a suitable environment for maintaining Leptospira spp. Hence, early detection of Leptospira spp. in the host
prompts control and protection, and the polymerase chain reaction (PCR) is a suitable method. The present report aimed to demonstrate the PCR analysis of bovine urine for detection of leptospiral DNA. A total of 98 urine samples were randomly collected from cattle bladder in Rasht abattoir of Iran and the presence of leptospiral DNA was assayed by PCR amplification of rrs (16S rRNA) gene and the results confirmed by nested PCR. Out of 98 urine samples in 42 samples leptospires DNA was identified with the frequency of 43%. The high presence of the organism in the urine of carriers is a serious threat to the dairy farms and to the public health which requires an effective control measure in the north provinces of Iran.

Keywords