The effect of short-term treatments of a gonadotropinreleasing hormone analog (buserelin) on sciatic nerve regeneration

Document Type: Full paper (Original article)

Authors

1 Neurophysiology Research Center, Department of Anatomy, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2 Department of Anatomy, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

3 Neurophysiology Research Center, Department of Physiology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

4 MD Student, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

5 Graduated from School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Abstract

Gonadotropin-releasing hormone (GnRH) has neuromodulatory roles in central and peripheral nervous systems. The purpose of this study is to evaluate the effect of GnRH analog (buserelin) on peripheral nerve regeneration. Forty adult male rats were divided into buserelin-treated, normal saline, sham surgery, and castrated + buserelin groups. The left sciatic nerve was crushed by a fine forceps and all animals were evaluated by sciatic functional index (SFI), electrophysiology, histology and immunohistochemistry
testing. On post operation days 21 and 28, the difference between buserelin and normal saline groups was statistically significant (P<0.05), but no significant difference was found between the buserelin and castrated + buserelin groups (P>0.05). At the 28th day after operation, the diameters (μm) of the regenerated myelinated fibers of the buserelin group were significantly greater than those of the normal saline group (P<0.05). Although nerve conduction velocity (NCV) of the buserelin group was faster than the normal
saline group, the difference was not statistically significant. The present study suggests that buserelin treatment might accelerate peripheral nerve regeneration.

Keywords