Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus

Document Type: Short paper

Authors

1 MVSc in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India

2 Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India

3 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388001, Gujarat, India

Abstract

Chicken anemia virus was detected by PCR in tissue samples collected from poultry flocks in Gujarat,
India. The VP1, VP2 and VP3 gene sequences of CAV from Anand, Gujarat were obtained after cloning the
PCR products in pDrive cloning vector. Nucleotide sequence alignment with other CAV sequences
demonstrated overall identity of 95-98.8%, 98.8-99.8% and 98.8-100% for VP1, VP2 and VP3 regions,
respectively. Deduced amino acid sequences revealed 91.7-99.7%, 99-100% and 97.3-100% homologies for
VP1, VP2 and VP3 proteins, respectively, indicating high level of genome conservation. Further, placement
of critical nucleotides and amino acids at particular positions indicated that Anand CAV is possibly of more
pathogenic potential. The CAV isolates were phylogenetically grouped together independent of their
geographic origin.

Keywords