Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells

Document Type: Short paper

Authors

1 Graduated from Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Department of Avian Medicine, Razi Vaccine and Serum Research Institute, Karaj, Iran

Abstract

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was cloned
in a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leader
sequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. The
construct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 was
assessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was a
neutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated the
construct was functional with respect to expression and secretion of a native VP2.

Keywords