Document Type: Short paper
Department of Food Hygiene and Public Health, School of Veterinary
Medicine, Shiraz University, Shiraz, Iran
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Ira
Prochymosin is one of the most important aspartic proteinases used as a milk-clotting enzyme in cheese production. In the present investigation we report sequence of cDNA encoding goat ( Capra hircus ) preprochymosin and compare its nucleotide and deduced amino acid sequences with sequences of other ruminants preprochymosin. As bovine prochymosin, the caprine prochymosin cDNA encodes 365 amino acids with a prosegment of 42 amino acids and the mature goat chymosin begins with glycine. The preprochymosin nucleotide sequence reported in this study differs from other reported goat sequence (AY389343) in three nucleotides, two of which alter the amino acids at positions 19p and 139.