SDS-PAGE analysis of urinary proteins in dogs with heartworm disease

Document Type: Short paper

Authors

1 Department of Veterinary Science, Institute of Biological Sciences, Autonomous University of Juarez, Ciudad Juárez, México

2 Department of Internal Medicine, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Las Palmas, Spain

3 Department of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain

Abstract

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.
Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinary
protein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins were
fractionated using SDS-PAGE. Results showed statistically significant differences for the U P/C ratio
(P<0.05) but not for USG and urinary protein concentrations (P>0.05) between groups. Urinary protein SDSPAGE analysis showed eight distinct bands in the urine of heartworm-infected dogs. The presence of proteins exclusively found in the urine of infected dogs suggests renal damage, even in cases of light proteinuria, indicating that SDS-PAGE is a sensitive method for the identification and characterisation of renal
proteinuria in dogs with heartworm disease.

Keywords