Document Type: Short paper
Department of Veterinary Science, Institute of Biological Sciences, Autonomous University of Juarez, Ciudad Juárez, México
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Las Palmas, Spain
Department of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain
The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.
Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinary
protein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins were
fractionated using SDS-PAGE. Results showed statistically significant differences for the U P/C ratio
(P<0.05) but not for USG and urinary protein concentrations (P>0.05) between groups. Urinary protein SDSPAGE analysis showed eight distinct bands in the urine of heartworm-infected dogs. The presence of proteins exclusively found in the urine of infected dogs suggests renal damage, even in cases of light proteinuria, indicating that SDS-PAGE is a sensitive method for the identification and characterisation of renal
proteinuria in dogs with heartworm disease.