1Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
2Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
3Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
This study was conducted to reveal the estrogenic effects of bisphenol A and o, p’-DDT on quail embryos. Thirteen fertilized eggs were used as control (injected with 20 μl corn oil), 15 eggs were injected with estradiol 17β (0.04 mg dissolved in 20 μl corn oil), 20 eggs were injected with BPA (2 mg dissolved in 20 μl corn oil) and 20 eggs were injected with o, p’-DDT (2 mg dissolved in 20 μl corn oil) at day 13 of incubation. Two days later the livers of the embryos were collected. The DNA was extracted from the liver for molecular sexing, while total RNA was extracted for vitellogenin II (VTGII) mRNA expression in embryos. In female embryos, BPA and o, p’-DDT induced variable levels of VTGII mRNA expression, while in male embryos, o, p’-DDT induced a slightly VTGII mRNA expression. In contrast, there was no expression of VTGII after BPA injection. In conclusion, the estrogenicity of BPA was lower than o, p’-DDT and both of them were lower than the estradiol 17β.