Genotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran

Document Type: Full paper (Original article)

Authors

1 Ph.D. Student in Bacteriology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatis
genotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attending
Shahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis by
plasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR amplification
and sequencing of 571 fragment encompassing VD1 and VD2 of omp1 gene. The overall prevalence rate of
C. trachomatis in endocervical specimens determined by plasmid nested PCR was 8%. The deduced serovars
found, in descending order of prevalence, were F (46.6%), E (33.3%), and D (13.3%), and serovar G was
found in a single sample. Sequence mutation analysis by BLAST search against GenBank reference
sequences identified 4 genetic variants. This study can be considered a contribution to increasing knowledge
on C. trachomatis genotype distribution and sequence variations within each genotype in Shiraz. Further
studies are needed to better define molecular epidemiology of C. trachomatis serovars and to investigate its
genotype variations in Iran.

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