Salmonella enterica serovar Enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination

Document Type: Full paper (Original article)

Authors

1 Department of Microbiology, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China

2 Agricultural Office of Dalingshan Town, Dongguan 523830, Guandong Province, China

Abstract

Serovar-specific real-time PCR for Salmonella enterica serovar Enteritidis (S. Enteritidis) was conducted
to detect the genomic DNA of S. Enteritidis from laying goose after subcutaneous vaccination at different
time points. Indirect fluorescent antibody (IFA) technique and immunohistochemical localization were
employed to validate the results. The results showed that S. Enteritidis was consistently detected in all the
samples. Vagina and uterus were positive at 20 h PI, and the last organ to show a positive result was the
largest and third largest preovulatory follicle, at 32 h PI. The copy numbers of S. Enteritidis DNA in each
tissue reached a peak at 36-60 h PI, with the vagina and uterus containing higher concentrations than other
tissues. However, the number of bacteria started decreasing by 3-4 d, and by 6 d, the concentration of S.
Enteritidis DNA was below the detection limits of the PCR assay, except the vagina. The real-time PCR
analysis of a variety of tissues is significant for further investigation of the mechanism of vaccine protection
and the optimization of vaccination regimes.

Keywords