Study on pattern of Neospora caninumtachyzoite proteins by SDS-PAGE and Western blotting in aborted cows

Document Type: Scientific report

Authors

1 Department of Pathobiology, Faculty of VeterinaryMedicine, University of Tabriz, Tabriz, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

Abstract

The intracellular parasite Neospora caninumis prevalent in several countries and is increasingly
recognized as an important cause of abortion and stillbirth in cattle. For characterizeing the tachyzoite
antigens of Neospora caninumin aborted cows, sera were obtained from 116 cows which were aborted in the
third semester of the pregnancy period and had antibodies to Neospora caninumin ELISA. To obtain the
protein content of Neospora, purified tachyzoites were lysed, electrophoretically separated and blotted to
nitrocelloluse membrane for immunostaining. Minimum 9 and maximum 13 protein bands ranging from 10
to 90 kDa were observed after immunostaining. It seems that, in almost all of the cows, two protein bands
with a molecular weight of 45 and 41 kDa, have a prominent reaction in Western blotting. According to our
findings, these two protein bands are the most important antigens observed after Western blotting, in
seropositive aborted cows.

Keywords