1Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Anatomical Sciences, School of Medicine, Jondishapoor University of Medical Sciences, Ahvaz, Iran
3Department of Aquatic Animal Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Physiology, School of Medicine, Jondishapoor University of Medical Sciences, Ahvaz, Iran
In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically. Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture media were collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically and incubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h at 20°C. Then, they were divided into two groups: control group which were incubated in BSS medium and experimental group which subdivided into three subgroups according to treatment with different concentration of collected pituitary secretion (50, 100 and 200 μl/ml). Follicles culture media were collected 24 h later and were analyzed for 17-β-oestradiol (E2) and 17-α-hydroxy progesterone (P4) content by radioimmunoassay (RIA). The results showed that adding low concentration (50 μl/ml) of collected pituitary secretion (CPS) increased steroid hormones (E2 and P4) secretion of incubated ovarian follicles significantly (P<0.05) but the high concentration of CPS (200 μl/ml) significantly decreased the secretion of E2 and P4 (P<0.05). Collected pituitary secretion at the concentration of 100 μl/ml had no significant effect on steroid hormones (P>0.05).