Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles

Document Type: Full paper (Original article)

Authors

1 Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Anatomical Sciences, School of Medicine, Jondishapoor University of Medical Sciences, Ahvaz, Iran

3 Department of Aquatic Animal Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Department of Physiology, School of Medicine, Jondishapoor University of Medical Sciences, Ahvaz, Iran

Abstract

In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.
Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture media
were collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically and
incubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h at 20°C. Then, they
were divided into two groups: control group which were incubated in BSS medium and experimental group
which subdivided into three subgroups according to treatment with different concentration of collected
pituitary secretion (50, 100 and 200 μl/ml). Follicles culture media were collected 24 h later and were
analyzed for 17-β-oestradiol (E2) and 17-α-hydroxy progesterone (P4) content by radioimmunoassay (RIA).
The results showed that adding low concentration (50 μl/ml) of collected pituitary secretion (CPS) increased
steroid hormones (E2 and P4) secretion of incubated ovarian follicles significantly (P<0.05) but the high
concentration of CPS (200 μl/ml) significantly decreased the secretion of E2 and P4 (P<0.05). Collected
pituitary secretion at the concentration of 100 μl/ml had no significant effect on steroid hormones (P>0.05).

Keywords