TY - JOUR ID - 74 TI - Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Bonyadian, M. AU - Moshtaghi, H. AU - Nematalahi, A. AU - Rahimi, E. AU - Akhavan Taheri, M. AU - Karami, S. AD - Department of Health and Food Quality Control, Institute of Zoonoses Research, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran AD - Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran AD - Graduated from Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran Y1 - 2011 PY - 2011 VL - 12 IS - 3 SP - 252 EP - 255 KW - Chicken carcasses KW - Escherichia coli KW - ETEC KW - EAggEC KW - PCR DO - 10.22099/ijvr.2011.74 N2 - The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains ofEscherichia coli in chicken carcasses by polymerase chain reaction (PCR). In this study 63 strains of E. coliwere isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughterhouse of Shahrekord. Polymerase chain reaction assays were used to detect the presence of the genesencoding heat-stable enterotoxin a (STa), heat-stable enterotoxin b (STb), heat labile toxin (LT) andEnteroaggregative heat-stable toxin 1 (EAST1). Sixty three out of 110 (57.27%) carcasses were contaminated with E. coli. Six out of 63 (9.52%) harbored the gene for LT, 1 (1.58%) STb, 21 (33.3%) EAST1 and 8 (12.69%) contain both LT and EAST1 genes. None of the strains contain the STa gene. The results indicated that contamination of the chicken carcasses with E. coli in such a level could be a potential hazard for consumers. UR - https://ijvr.shirazu.ac.ir/article_74.html L1 - https://ijvr.shirazu.ac.ir/article_74_db7506c8789eb3a83d06fddbd9c2460e.pdf ER -