TY - JOUR ID - 53 TI - The relation of water contamination and Colibacillosis occurrence in poultry farms in Qom province of Iran JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Karimi, V. AU - Zahraei Salehi, T. AU - Sadegh, M. AU - Jaafarnejad, S. AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Y1 - 2011 PY - 2011 VL - 12 IS - 2 SP - 133 EP - 138 KW - Colibacillosis KW - Drinking water KW - PCR KW - Poultry farms KW - Qom province DO - 10.22099/ijvr.2011.53 N2 - Seventy poultry farms’ drinking water was tested for Escherichia coli contamination in Qom province inIran. The cases of colibacillosis from positive farms were also collected and tested. The isolates wereexamined for serotype, detection of virulence genes by multiplex PCR and antibiotic resistance. Thirtypoultry farm water samples were E. coli positive (18.57%), although 13 E. coli isolates were recovered fromcarcasses of related farms. The isolates belonged to O2 serogroup and one O157, with approximately 29% of the strains being non-typeable. Two isolates from water and carcasses were serotyped O2 and one  sample serotyped O157, which needs to be further studied. The PCR method was on the basis of showing virulence genes of espB, stx1, stx2 and eae. One sample from water and one from a carcass were shared espB, stx2 and eae genes. Stx1 and stx2 genes were common in a sample from both water and carcass, although five samples from both water and carcass shared a stx1 gene as well. All isolates showed maximum sensitivity and resistance to lincospectine and tetracycline, respectively. UR - https://ijvr.shirazu.ac.ir/article_53.html L1 - https://ijvr.shirazu.ac.ir/article_53_d8f4fef2fec7755084984a466ec31eb1.pdf ER -