TY - JOUR ID - 3813 TI - Proteomic analysis of muscle tissue from rainbow trout (Oncorhynchus mykiss) fed dietary β-glucan JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Ghaedi, Gh. AU - Keyvanshokooh, S. AU - Mohammadi Azarm, H. AU - Akhlaghi, M. AD - Graduated from Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran AD - Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran AD - Department of Aquatic Animal Health and Diseases, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Y1 - 2016 PY - 2016 VL - 17 IS - 3 SP - 184 EP - 189 KW - Dietary β-glucan KW - Growth KW - Proteomics KW - Rainbow trout DO - 10.22099/ijvr.2016.3813 N2 - The aim of this study was to examine the changes in muscle proteome of the rainbow trout fed dietary β-glucan. The experimental diets contained 0 (control), 0.1% and 0.2% β-1,3/1,6 yeast glucan. First, feeding larvae were fed to apparent satiation nine times per day with their respective diets over two months. The percentage of body weight gain and feed efficiency of fish fed 0.2% diet was significantly higher than those of fish fed the control and 0.1% diets. Fish fed the control and 0.2% diets were subjected to proteomic analysis. Proteins of the muscle tissue were analyzed using two-dimensional electrophoresis and mass spectrometry. Spots that were found to differ significantly in abundance between control and β-glucan fed fish were selected for identification. Out of 8 protein spots showing differential expression, 7 spots were successfully identified. Two protein spots that were found to be increased in abundance in the β-glucan treated rainbow trout corresponded to tropomyosin alpha-1 chain (spot 1) and slow myotomal muscle tropomyosin (spot 2). The five spots that were down-regulated with dietary β-glucan supplementation were identified as different forms of myosin: myosin light polypeptide 3-2 (spot 3), myosin light chain 1 (spots 4 and 5), fast myosin light chain 2 (spot 6) and myosin heavy chain (spot 7). The altered expression of structural proteins in fish fed β-glucan may be related to higher growth rate in rainbow trout. These findings provide basic information to understand possible mechanisms of dietary β-glucan contribution to better growth in rainbow trout. UR - https://ijvr.shirazu.ac.ir/article_3813.html L1 - https://ijvr.shirazu.ac.ir/article_3813_8043ca01e4556fe56cd4e7aecbe54423.pdf ER -