TY - JOUR ID - 3812 TI - PFGE genotyping and molecular characterization of Campylobacter spp. isolated from chicken meat JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Bakhshi, B. AU - Kalantar, M. AU - Rastegar-Lari, A. AU - Fallah, F. AD - Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran AD - Pediatric Infection Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Y1 - 2016 PY - 2016 VL - 17 IS - 3 SP - 177 EP - 183 KW - Antimicrobial resistance KW - Campylobacter spp KW - Integron KW - Pulsed-field gel electrophoresis DO - 10.22099/ijvr.2016.3812 N2 - A total of 70 samples were collected from chicken meat obtained from 10 markets in Tehran, Iran from which 39 Campylobacter coli were isolated. Among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (SXT) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). No resistance was to gentamycin was observed. None of the Campylobacter strains under study harbored integron, suggesting the involvement of other resistancemechanisms in emergence of multi drug resistance (MDR) phenotype among the isolates. Two major types (A and B) and 15 subtypes (A1-A8 and B1-B7) were identified. Pulsed-field gel electrophoresis (PFGE) analysis demonstrated a high degree of homogeneity while the majority of the isolates shared identical or very similar PFGE genotypes. Isolates with identical genotypes differed in their resistance profile, although all of them assigned to MDR phenotype. To our knowledge, this is the first molecular survey from Iran characterizing Campylobacter isolates from poultry, which adds to our knowledge the epidemiological linkage ofCampylobacter isolates with MDR properties from different sources and emphasizes the need for cautious use of antimicrobials in different fields of food production chain. UR - https://ijvr.shirazu.ac.ir/article_3812.html L1 - https://ijvr.shirazu.ac.ir/article_3812_c8a278de484849c18211c55b15bbb5f8.pdf ER -