TY - JOUR ID - 3292 TI - Novel polymorphism of AA-NAT gene in Indian goat breeds differing in reproductive traits JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Sharma, R. AU - Ahlawat, S. AU - Tantia, M. S. AD - Core Lab, National Bureau of Animal Genetic Resources, Karnal-132001, India Y1 - 2015 PY - 2015 VL - 16 IS - 4 SP - 377 EP - 380 KW - Arylalkylamine-N-acetyltransferase (AA-NAT) gene KW - Goat KW - Polymorphism DO - 10.22099/ijvr.2015.3292 N2 - This is the first description of the polymorphisms of arylalkylamine-N-acetyltransferase (AA-NAT) gene in Indian goats with different reproductive traits (twinning percentage and age of sexual maturity). Based on the important role of AA-NAT in reproduction, it is considered as a possible candidate gene for this trait. Two novel synonymous SNPs, C825T (exon2) and C1249T (exon3) were identified. All three possible genotypes (CC, CT and TT) were identified for C825T mutation whereas two genotypes were observed (CC and CT) for C1249T mutation. SNPs C825T and C1249T changed recognition site of restriction enzyme BtsCI (GGATG) and AciI (CCGC) and thus can be genotyped by the relatively simple and cost effective technique of PCR-RFLP for establishing further association with reproductive traits. Present results add to the meager existing knowledge and extend the spectrum of genetic variation of caprine candidate genes of reproductive traits, which is another step towards improvement of goat genetic resources and breeding. UR - https://ijvr.shirazu.ac.ir/article_3292.html L1 - https://ijvr.shirazu.ac.ir/article_3292_fd1d3002ed735eec3746329d3514cbc0.pdf ER -