TY - JOUR ID - 3072 TI - Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Awad, A. AU - Khalil, S. R. AU - Abd-Elhakim, Y. M. AD - Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt AD - Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, 44511 Zagazig, Egypt Y1 - 2015 PY - 2015 VL - 16 IS - 2 SP - 218 EP - 222 KW - Avian species KW - Cytochrome b gene KW - Phylogenetic analysis DO - 10.22099/ijvr.2015.3072 N2 - Veritable identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. Therefore, this study involved the application of molecular approach to identify some avian species i.e. Chicken (Gallus gallus), Muskovy duck (Cairina moschata), Japanese quail (Coturnix japonica), Laughing dove (Streptopelia senegalensis), and Rock pigeon (Columba livia). Genomic DNA was extracted from blood samples and partial sequence of themitochondrial cytochrome b gene (358 bp) was amplified and sequenced using universal primers. Sequences alignment and phylogenetic analyses were performed by CLC main workbench program. The obtained five sequences were deposited in GenBank and compared with those previously registered in GenBank. The similarity percentage was 88.60% between Gallus gallus and Coturnix japonica and 80.46% between Gallus gallus and Columba livia. The percentage of identity between the studied species and GenBank species ranged from 77.20% (Columba oenas and Anas platyrhynchos) to 100% (Gallus gallus and Gallus sonneratii, Coturnix coturnix and Coturnix japonica, Meleagris gallopavo and Columba livia). Amplification of the partial sequence of mitochondrial cytochrome b gene proved to be practical for identification of an avian species unambiguously. UR - https://ijvr.shirazu.ac.ir/article_3072.html L1 - https://ijvr.shirazu.ac.ir/article_3072_7ed87d1be0e4c027f56c757700e1afe3.pdf ER -