TY - JOUR ID - 2664 TI - Detection of hydatid fluid and protoscolices antigens in sheep with hydatidosis JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Rajabiyoun, M. AU - Hashemitabar, Gh. R. AU - Tavakool Afshari, J. AD - Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran AD - Department of Immunology, Booali Institute, University of Medical Sciences of Mashhad, Mashhad, Iran Y1 - 2006 PY - 2006 VL - 7 IS - 2 SP - 59 EP - 64 KW - Hydatidosis KW - Hydatid fluid KW - Protoscolex KW - SDS-PAGE KW - Western blotting DO - 10.22099/ijvr.2006.2664 N2 - Hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,Echinococcus granulosus. This is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. In the current study, 20 sheep cystic livers or lungs were collected.Hydatid fluid and protoscolices were isolated and the soluble protein was prepared. Polypeptide profile ofhydatid fluid and protoscolices was analysed by SDS-PAGE with 12.5% acrylamide concentration. Humoralimmunity and antigenic pattern were evaluated by Western blotting. In electrophoresis of hydatid fluid, fivebands with molecular weight of 84, 68–70, 55, 27–28 and 16–17 kDa were observed; the 68–70 and 55 kDabands were noticeably dominant. In Western blot of hydatid fluid protein, only 68–70 and 55 kDa bands hadconciderable positive reaction. A total of 12 bands were also observed in protoscolices polypeptide profile.The molecular weight of the bands were 120, 88–89, 84, 66, 55–56, 49–50, 43–44, 36, 30–31, 24, 20 and 15–16 kDa. Seven bands with molecular weight of 66, 55–56, 49–50, 43–44, 36, 30–31 and 24 kDa had positive reaction in Western blot. We concluded that the determination of specific antigenic bands for sheep hydatid fluid and protoscolices was successfully achieved in this study. UR - https://ijvr.shirazu.ac.ir/article_2664.html L1 - https://ijvr.shirazu.ac.ir/article_2664_7e75e2b9605f01b0206c9d9d24702b64.pdf ER -