TY - JOUR ID - 2619 TI - Immunization of Arabian sheep with whole gut homogenate of Haemonchus contortus JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Nayebzadeh, H. AU - Seyfiabad Shapouri, M. R. AU - Hoghooghi-Rad, N. AU - Masouri, B. AD - Department of Parasitology, College of Veterinary Medicine, Lorestan University, Lorestan, Khorramabad, Iran AD - Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran AD - Department of Parasitology, Faculty of Specialized Sciences of Veterinary Medicine, Science and Research Campus, Islamic Azad University, Tehran, Iran AD - Department of Parasitology, College of Veterinary Medicine, Lorestan University, Lorestan, Khorramabad, Iran; Y1 - 2008 PY - 2008 VL - 9 IS - 4 SP - 360 EP - 364 KW - Haemonchus contortus KW - Vaccination KW - Whole gut homogenate KW - Arabian sheep DO - 10.22099/ijvr.2008.2619 N2 - Ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free conditions. Animals in group 1 were immunized two times by whole gut homogenate (WGH) of Haemonchus contortus emulsified in Freund’s adjuvant. In group 2 (control), animals were injected by phosphate buffered saline emulsified in the same adjuvant. Animals were challenged by 10000 third-stage larvae (L3) of Haemonchus contortus on day 33 after the first immunization and then humanely killed on day 42. Animals were tested for serum antibody and eggs per gram of faeces (EPG) throughout the study and nematodes in their abomasom were counted after necropsy. The results indicated that animals immunized with WGH showed a higher level of serum antibodies. A significant difference was observed in mean optical density of sera in ELISA between the two groups (P<0.05) and a 77 and 78% reduction in EPG and nematode counts at necropsy, respectively (P<0.05). UR - https://ijvr.shirazu.ac.ir/article_2619.html L1 - https://ijvr.shirazu.ac.ir/article_2619_8b97b5712d1d2d50d574a4134922f9db.pdf ER -