TY - JOUR ID - 2533 TI - Phenotypic and genetic diversity of motile aeromonads isolated from diseased fish and fish farms JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Modarres Mousavi Behbahani, S. M. AU - Akhlaghi, M. AU - Sharifiyazdi, H. AD - Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran AD - Aquatic Animal Health Unit, School of Veterinary Medicine, Shiraz University, Shiraz, Iran AD - Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Y1 - 2014 PY - 2014 VL - 15 IS - 3 SP - 238 EP - 243 KW - Phenotype KW - Genotype KW - Motile aeromonads KW - Diseased fish KW - Fish farms DO - 10.22099/ijvr.2014.2533 N2 - Samples from the kidney of 100 diseased fish with signs of haemorrhagic septicemia and 50 samples from outlet water of fish farms were taken aseptically and cultured. In the laboratory, 75 colonies of gram-negative bacteria were biochemically diagnosed as motile aeromonads in our Bacteriology Laboratory Unit using API 20E rapid identification system. The genotype identification using specific primers for 16S rDNA by PCR and direct sequencing of 28 Iranian motile aeromonads isolates were as follow: in diseased fish, Aeromonas hydrophila (3 isolates, 15%), A. veronii bv. sobria (8 isolates, 40%), A. bestiarum/piscicola (5 isolates, 25%), A. media (3 isolates, 15%), A. jandaei (1 isolate, 5%), A. aquariorum (0 isolate, 0%) and in water, Aeromonas hydrophila (0 isolate, 0%), A. veronii bv. sobria (6 isolates, 75%), A. bestiarum/piscicola (0 isolate, 0%), A. media (1 isolate, 12.5%), A. jandaei (0 isolate, 0%) and A. aquariorum (1 isolate, 12.5%). Results of this study suggest that the incidence of motile aeromonads septicemia due to A. veronii bv. sobria is the most prevalent motile aeromonads. Nucleotide polymorphisms on the sequencing results of the 16S rDNA were detected as noticeable inter and intra-specific variation within the population of different aeromonads isolates. In total, 10-20 variant nucleotide positions in this region were observed among Aeromonas spp. UR - https://ijvr.shirazu.ac.ir/article_2533.html L1 - https://ijvr.shirazu.ac.ir/article_2533_b41136bd64ff20577c0d12ee8cde2de1.pdf ER -