TY - JOUR ID - 1827 TI - The effect of seminal plasma on the quality of coated ram frozen-thawed spermatozoa JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Roostaei-Ali Mehr, M. AU - Sharafi, F. AD - Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran AD - Graduated from Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran Y1 - 2013 PY - 2013 VL - 14 IS - 4 SP - 305 EP - 312 KW - Cryopreservation KW - Sperm coating KW - Seminal plasma KW - Ram KW - Spermatozoa DO - 10.22099/ijvr.2013.1827 N2 - Two experiments were designed to examine the effects of crude seminal plasma (CSP) exposure ofejaculated and epididymal spermatozoa before freezing. Experiment 1, two consecutive ejaculates werecollected (n=4) within 5 min, the second one was carried out in a tube containing Fiser extender (coatedspermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. The pellets were split into three parts and 0, 50 and 100% CSP (v/v) was added and they were frozen. Samples were thawed and incubated at 37°C for 6 h. The highest progressive motility (10.41%) and the highest hypo-osmotic responses (12.62%) were found for the coated spermatozoa without CSP at 6 (P<0.05). The maximum viability was found for coated spermatozoa without CSP at 0 (29.4%) and 6 (17%, P<0.05). Experiment 2, epididymal spermatozoa were recovered (n=6), pooled and split into three fractions and 0, 50 and 100% CSP and the diluents were added, after that they were frozen. Thawed epididymal spermatozoa were incubated at 37°C for 6 h. The highest (33.3%) and lowest (25%) progressive motility were found for the epididymal spermatozoa without CSP and the epididymal spermatozoa with 100% CSP at 0, respectively (P<0.05). The highest (19.37%) and the lowest (9.38%) viability were related to the epididymal spermatozoa with 0 and 100% CSP at 6, respectively (P<0.05). Under the conditions of the current study, the addition of CSP to the ram epididymal and coated spermatozoa strengthened the detrimental effect of the freezing procedure. UR - https://ijvr.shirazu.ac.ir/article_1827.html L1 - https://ijvr.shirazu.ac.ir/article_1827_839bc1d79d0b4ae205573112a03f1db2.pdf ER -