TY - JOUR ID - 181 TI - Bacteriological study of dead-in-shell embryos of ostrich JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 Y1 - 2010 PY - 2010 VL - 11 IS - 1 SP - 88 EP - 90 KW - Bacterial isolates KW - Ostrich egg KW - Yolk sac infection DO - 10.22099/ijvr.2010.181 N2 - In order to study the bacterial flora of dead-in-shell ostrich chicks, twelve unhatched eggs which did nothave external pipping during the hatching period were transferred to the laboratory of microbiology. The eggshells were accurately disinfected and the embryos were removed and placed in a sterile plate. The surface of each embryo was swabbed with a sterile swab which was also plunged through the yolk sac and the embryo contents and the swab were inoculated into tryptic soy broth (TSB) or nutrient broth. To enrich Salmonella spp., another swab was prepared as above and inoculated into Selenite-F broth. These media were incubated at 37°C for 24 h and then subcultured by streak plate method on solid media. Different bacterial colonies on solid media were isolated in pure cultures for further identification. The results of this study showed that the predominant bacterial flora of dead-in-shell embryos of ostrich were Bacillus spp. (45%) and Staphylococcus spp. (25%). UR - https://ijvr.shirazu.ac.ir/article_181.html L1 - https://ijvr.shirazu.ac.ir/article_181_b35fbf81b9bb05a7b52629015c1a3311.pdf ER -