TY - JOUR ID - 1681 TI - Immune efficacy of OmpH and OmpA DNA vaccines against avian Pasteurella multocida JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Gong, Q. AU - Qin, C.L. AU - Niu, M.F. AU - Cheng, M. AU - Sun, X. F. AU - Zhang, A.G. AD - Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China AD - MSc in Molecular Biology, Department of Biological Engineering, School of Food and Biological Engineering, He Nan University of Science and Technology, Luoyang, 471003 PR, China Y1 - 2013 PY - 2013 VL - 14 IS - 3 SP - 197 EP - 202 KW - Avian Pasteurella multocida KW - pOMPH KW - pOMPA KW - DNA vaccine KW - Immune efficacy DO - 10.22099/ijvr.2013.1681 N2 - Avian Pasteurella multocida is an agent of fowl cholera. The protective effect achieved through orthodoxvaccines is not ideal. The research on novel vaccines against avian Pasteurella multocida is imperative. Inthis study, the genes encoding outer membrane protein H and A (OmpH and OmpA) were cloned into theeukaryotic expression vector pcDNA3.1(+) and the recombinant plasmids, namely DNA vaccines (pOMPH andpOMPA) were obtained. Five groups of chickens (n=20 per group) were intramuscularly injected with thetwo recombinant plasmids, attenuated live vaccine, control vector pcDNA3.1(+) and PBS, respectively. Theimmune responses and protective efficacy were evaluated after immunization by serological and challenging.A significant increase in serum antibody levels was observed in chickens vaccinated with the attenuated livevaccine and the two DNA vaccines. Additionally, the lymphocyte proliferation (SI values) were higher inchickens immunized with the attenuated live vaccine and the two DNA vaccines than in those vaccinatedwith pcDNA3.1(+) and PBS (P<0.05). Furthermore, the two DNA vaccines provided partial protection to thevaccinated chickens; however, the protective efficacy was inferior to that provided by the attenuated livevaccine. UR - https://ijvr.shirazu.ac.ir/article_1681.html L1 - https://ijvr.shirazu.ac.ir/article_1681_2d89e86e3bc8ea26ddf375a20df9ec7d.pdf ER -